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PCR/qPCR Data Analysis
After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.
KAPA Express Extract Kit FAQs
Frequently asked questions (FAQs) for KAPA Express Extract Kit.
Introduction and Historical Timelines
Learn about the history of the polymerase chain reaction (PCR), from the basic principles that proceeded its discovery to the awarding of a Nobel Prize for Chemistry and more recent developments such as real-time PCR (qPCR) and digital PCR.
Restorase® DNA Polymerase
Restorase® was developed for researchers unable to achieve amplification of damaged DNA templates when using other commercially available DNA polymerases.
Extract-N-Amp™ Tissue Feature Article
Standard methods for extracting DNA from tissues can be extremely laborious and time consuming. Certain applications, such as genotyping of transgenic mice using a section of tail, employ a lengthy DNA extraction process.
GenomePlex® Whole Genome Amplification Kit: A High-Throughput Approach for Rapidly Amplifying Genomic DNA from a Variety of Biological Materials
GenomePlex® Whole Genome Amplification Kit: A High-Throughput Approach for Rapidly Amplifying Genomic DNA from a Variety of Biological Materials
MIQE – Minimum Information for Publication of Quantitative Real-Time PCR Experiments
Our qPCR services, including primer and probe designs, assay protocol development, troubleshooting, and data analysis support, adhere to MIQE, which allows you to publish or bring your product to market faster and with confidence.
Extract-N-Amp Reagent Provides a Highly Effective Way of Generating PCR Products from FTA® Blood Cards
Extract-N-Amp is a versatile, combined DNA extraction and amplification kit intended to simplify the generation of PCR products from a range of sample types. This methodology can be used without modification to perform PCR on blood samples stored on FTA
PCR Assay Optimization and Validation
PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.
PCR/qPCR/dPCR Assay Design
The entire PCR workflow is vulnerable to factors which introduce variability. Many of the variable components are unavoidable, such as the source of the sample or the requirement for a reverse transcription step. Assay design is also highly variable and
Extract-N-Amp™ Product Guide
Extract-N-Amp™ PCR kits are the world’s first integrated extraction and amplification process for rapid blood, tissue or plant assays.
Colony PCR
Colony PCR reagents and our colony PCR protocol using REDExtract-N-Amp™ PCR ReadyMix™ and JumpStart™ REDTaq® PCR ReadyMix™ reagents.
Locked Nucleic Acid
Frequently asked questions about Locked Nucleic Acids (LNA)
ThermaStop™-RT PCR Additive
PCR additive interacts with reverse transcriptase at low temperatures to reduce priming problems that lead to nonspecific bands.
KAPA2G Robust PCR Kits FAQs
Frequently asked questions (FAQs) for KAPA2G Robust PCR Kits.
KAPA2G Fast PCR Kits FAQ
Fast PCR is PCR in which total reaction time is decreased by reducing the duration of one or more of the steps during each cycle of a PCR. Using the KAPA2G Fast PCR Kit, PCR time can be reduced by
RT-PCR / RT-qPCR Troubleshooting
Developing a PCR or RT-PCR/RT-qPCR troubleshooting protocol so that data are reliable is essential. Potential sources of RT-PCR or PCR error and problems include operator error, the PCR master mix, and oligo design. This PCR troubleshooting guide outlines and details
Technical Guide to PCR Technologies
Examples of basic PCR/qPCR/dPCR protocols that can be used as the foundation for explorations into some of the concepts described in the theoretical chapters of this guide. Included are detailed protocols for assay quality control, in addition to more general
ThermaStop™ PCR Additive
PCR additive added to master mix that stops nonspecific bands after cool down.
Whole Transcriptome Amplification of RNA from Low Cell-Number Samples
The efficacy of amplification of small quantities of total RNA with the Complete Whole Transcriptome Amplification Kit (WTA2) was examined in this study.
GC-RICH PCR System Troubleshooting
GC-RICH Amplification of Polymerase Chain Reaction (PCR) System Troubleshooting.
Troubleshooting PCR and RT-PCR Amplification
This page shows PCR and RT-PCR amplification troubleshooting.
Long and Accurate PCR
Long and accurate PCR applications address the needs for longer read lengths, greater fidelity and higher yields than that which can be achieved with Taq DNA polymerase.
Working with PCR
General Considerations; Factors to Consider in RT-PCR; Prevention of Carryover Contamination; Troubleshooting
PCR Master Mix
A PCR master mix is a batch of PCR or RT-PCR reagents that can be divided among many PCR reaction tubes. It usually includes DNA polymerase, dNTPs, MgCl2 and buffer. Make your own master mix or choose a commercial one.
Qualitative multiplex PCR assay for assessing DNA quality from FFPE tissues and other sources of damaged DNA
The assessment of DNA quality is a crucial first step in acquiring meaningful data from formalin-fixed paraffin-embedded (FFPE) tissues, and other sources of damaged DNA. Using intact genomic DNA is key for successful analysis of chromosomal aberrations (e.g. SNP analysis
Polymerase Chain Reaction (PCR) Basics
Polymerase chain reaction (PCR) is a technique that results in exponential amplification of a target DNA sequence.
Whole Genome Amplification for Single Cell Biology
Whole genome amplification (WGA) offers a means to overcome the above restrictions for single-cell genomic analyses. WGA has been described as a non-specific amplification technique that affords an amplified product completely representative of the initial starting material.
Quantitative PCR and Digital PCR Detection Methods
Fluorescent dyes or probes are included in PCR mixes to monitor the change in NA amplicon concentration as the reaction proceeds.
Complete Solutions for PCR Assay Development
Fit-for-use products offer the quality, consistency & documentation necessary for every step of your IVD development and manufacturing process.
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