Merck
Search Within
Applications
Content Type

Protein lysis and extraction

Applied Filters:
facet applications:Protein lysis and extraction
facet content type:Protocol
Protease Inhibition Using Protease Inhibitor Mix
Protease Inhibitor Mix from Cytiva inhibits serine, cysteine, metalloproteases, and calpain proteases and is suitable for animal and plant tissues, yeast, and bacteria.
cOmplete™, Mini, EDTA-free Protocol
Dissolve one tablet in 10 ml aqueous buffer or water. If high proteolytic activity is present, dissolve one tablet in 7 ml buffer.
Cell Lysis and Protein Extraction for Western Blotting
Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.
Liberase™ DH Research Grade Protocol & Troubleshooting
Your current tissue dissociation protocol, and the extent to which you have been successful with traditional collagenase are good guides for selecting the appropriate Liberase Enzyme Blend.
Liberase™ DL Research Grade Protocol & Troubleshooting
Your current tissue dissociation protocol, and the extent to which you have been successful with traditional collagenase are good guides for selecting the appropriate Liberase Enzyme Blend.
Liberase™ TL Research Grade Protocol & Troubleshooting
Liberase™ TL Research Grade Protocol & Troubleshooting
Handling Inclusion Bodies in Recombinant Protein Expression
How to isolate proteins from inclusion bodies using Cytiva products.
Cell Disruption and Membrane Preparation
This page shows how to perform a cell disruption und prepare membranes with products from Cytiva.
Processing Small Tissue or Cell Samples using Sample Grinding Kit
Sample Grinding Kit from Cytiva is designed to disrupt cell or tissue samples for protein extraction.
Liberase™ TH Protocol & Troubleshooting
Liberase Enzyme Blend selection depends on tissue dissociation protocol and success with collagenase.
TRI Reagent® Protocol
TRI Reagent enables simultaneous DNA, RNA, and protein isolation with sample prep guidelines and troubleshooting.
Detergent-Free Nuclear Protein Extraction
Detergent-free procedure is recommended for nuclear protein preparation to avoid interference with labeling efficiency of extracted proteins.
Lysosome Isolation Kit
Lysosome Isolation Kit isolates lysosomes from tissues and cells for research purposes using differential centrifugation methods.