Many cultures obtained from a culture collection, such as ECACC, will arrive frozen and in order to use the cells they must be thawed and put into culture. It is vital to thaw cells correctly in order to maintain the viability of the culture and enable the culture to recover more quickly. Some cryoprotectants, such as DMSO, are toxic above 4 °C. Therefore, it is essential that cultures are thawed quickly and diluted in culture medium to minimize the toxic effects.
Watch this tutorial to learn best practices to start a fresh cell culture from frozen stocks, cell thawing tips, and tricks to maximize the survival of your cultures.