Blood cards provide the convenience of archiving small volumes of blood. However, many times genomic DNA from these samples is limited, which may hinder the researcher’s ability to perform downstream analysis. This protocol provides a simple and convenient method to extract genomic DNA from a blood card. Once the DNA has been extracted, it can then be amplified using the GenomePlex® Whole Genome Amplification protocol.
The GenElute Blood Genomic DNA Kit (Product No. NA2000) is recommended for this procedure.
Legend: Products were amplified using the GenomePlex Whole Genome Amplification Kit (Product No. WGA1) from Sigma, Supplier A’s kit and Supplier Q’s kit. Products were resolved on a 1.5% agarose gel. 5 µL of amplified product was added to each well. The products amplified using GenomePlex technology were of a smaller molecular weight as shown on the gel when compared to Supplier A and Q. This is due to the random fragmentation of genomic DNA prior to amplification. Sigma’s amplified products are specific and there is no amplicon visible in the negative control (lane 2) indicating that only the desired genomic DNA is amplified. Both Suppliers A and Q yield a nonspecific signal in the negative control which is equal in size and intensity to the signal for the suppliers’ positive control.
Lane 1—1 kb Marker
Lane 8—Supplier A Blood Card
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