ALiCE® Tubes or 96 half-well plate with lid
Thaw DNA template at room temperature, mix briefly and do not heat-treat.
Positive control plasmids pALiCE01 and pALiCE02 are supplied ready-to-use at 250 ng/µL. See instruction manual for additional information on template preparation.
Thaw the ALiCE® reaction mix in a water bath at room temperature (20 - 25 °C). Do not vortex ALiCE® Reaction mix! Place on ice directly after thawing. Start reactions within 30 min after thawing.
Freeze remaining lysate at -80 °C. Do not use liquid nitrogen. Avoid more than one freeze-thaw cycle.
Note: When using pALiCE plasmids with different inserts, dilute or concentrate to the same final molarity of 5 nM. DNA concentration may significantly affect protein yield.
* For best results, use plates from Greiner Bio-One, Art. No. 675086
Due to the long incubation time, evaporation may occur. Please refill the reaction vessel with RNase-free water to 50 µL after the reaction is completed.
For in vitro / research use only.
The kit is shipped frozen on dry ice, please check if the contents are still frozen upon delivery. Contact us immediately if any issues with delivery have occurred.