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SRE0093

Sigma-Aldrich

β-葡萄糖醛酸酶 来源于帽贝 (Patella vulgata)

aqueous solution

别名:
β-葡萄糖醛酸苷酶
CAS号:
MDL编号:
NACRES:
NA.54

质量水平

200

重组

expressed in proprietary host

形式

aqueous solution

浓度

100000-200000 units/mL

运输

ambient

储存温度

2-8°C

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应用

据报道,来自欧洲帽贝的酶比来自盖罩大蜗牛、牛肝脏和大肠杆菌的酶能更有效地水解阿片肽-葡糖苷酸
比较不同酶性能的新技术文章
了解更多关于 Sigma R&D 生成的最新应用数据 使用不同来源的酶优化不同药物类别的水解,并使用色谱纯化酶降低酯酶活性的影响,导致 6-MAM 转化为吗啡
Poster presentation comparing the hydrolysis efficiency of novel beta-glucuronidase enzymes from recombinant limpet and recombinant E. coli sources, click at the following poster link: Hydrolysis Efficiency Evaluation of Novel Recombinant Limpet and E. coli Beta-Glucuronidase Enzymes
More on the suitability of this product for hydrolysis of different drug-glucuronide substrates is available at this page.

特点和优势

Recombinantly expressed and purified to eliminate MAM esterase activity (6-Monoacetylmorphine→morphine). Representative analysis at high enzyme titer (50 un/μL β-glucuronidase) was less than 1% conversion of 6-MAM to morphine after 4 hours at 60 °C.

单位定义

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay).

外形

Supplied as a solution in 100mM potassium acetate pH 5.2. It is free of detergents, carbohydrates or any other components that may interfere with sample preparation and analysis.

储存分类代码

12 - Non Combustible Liquids

闪点(F)

Not applicable

闪点(C)

Not applicable

分析证书

原产地证书 (CofO)

技术文章

Evaluation of Different Enzymes on Hydrolysis Efficiencies of Glucuronide Drug Metabolites in Urine

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

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