The 3D perfusion bioreactor consists of multiple independent, autoclavable polycarbonate chambers. The chambers are interchangeable and are specially designed to take advantage of 3D Insert™ scaffolds with various sizes ranging from 96-well to 6-well. Cell culture medias are perfused through the open porous structure of scaffolds using a pulsatile pump. The entire unit is autoclavable (except pump, of course) and can be used as a single-use bioreactor system.
Numerous studies have proven that cells grow better under dynamic culture condition, simply because both nutrition supply and metabolic waste removal are better under dynamic culture conditions. Additionally, in some instances, the shear force produced by the flowing medium can act as a mechanical stimuli signal that will further promote stem cell differentiation toward certain cell lineage, such as osteoblast. Combined with the 3D Insert™ scaffold technology, the 3D Perfusion Bioreactor can facilitate the promotion of stem cell growth and differentiation, thereby enhancing stem cell research.