Merck
所有图片(1)

03-182

Sigma-Aldrich

HDAC2 (1-488) (His-tag) human recombinant

Human recombinant HDAC2 (1-488) (His-tag) produced in insect cells.

登录to View Organizational & Contract Pricing

Select a Size

eCl@ss:
32160405
NACRES:
NA.32

生物来源

human

质量水平

specific activity

>5 units/μg

分子量

Mw ~55 kDa

manufacturer/tradename

Upstate®

浓度

>50% (SDS-PAGE)

technique(s)

activity assay: suitable

运输

ambient

相关类别

比较类似商品

查看完整比较结果

显示差异

1 of 4

此商品
06-72017-10237AV32618
Anti-HDAC1 Antibody Upstate®, from rabbit

Sigma-Aldrich

06-720

抗HDAC1抗体

specific activity

>5 units/μg

specific activity

-

specific activity

-

specific activity

-

mol wt

Mw ~55 kDa

mol wt

-

mol wt

-

mol wt

55 kDa

concentration

>50% (SDS-PAGE)

concentration

-

concentration

-

concentration

0.5 mg - 1 mg/mL

technique(s)

activity assay: suitable

technique(s)

activity assay: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

ChIP: suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

shipped in

ambient

shipped in

ambient

shipped in

dry ice

shipped in

wet ice

一般描述

Histone deacetylase 2 (HDAC2), or transcriptional regulator homolog RPD3 L1, is highly homologous to the yeast transcription factor RPD3 (reduced potassium dependency 3) gene. As in yeast, human HDAC2 is likely to be involved in regulating chromatin structure during transcription. It associates with YY1, a mammalian zinc-finger transcription factor, which negatively regulates transcription by tethering RPD3 to DNA. This process is highly conserved from yeast to human.

应用

HDAC deacetylase assays
Human recombinant HDAC2 (1-488) (His-tag) produced in insect cells.
Research Category
Epigenetics & Nuclear Function

单位定义

Specific Activity: 1U = 1 pmol/min at 37°C using a standard flourometric activity assay to measure deacetylation activity.

外形

Format: Purified
In liquid containing 50 mM Tris, pH 8.0, 138 mM NaCl and 10% glycerol. Frozen solution.

储存及稳定性

Store at -80°C. The enzyme is stable on ice for 30 to 60 min, but may lose activity with dilution and/or prolonged storage on ice. It is recommended that thawing and addition of the enzyme to assay wells be done within as short a time as possible before start of the assay and that the thawed enzyme be kept on ice throughout. Best performance will be obtained by adding the chilled, undiluted enzyme directly to pre-warmed buffer and proceeding immediately to the addition of pre-warmed substrate. The remaining, unused enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. The enzyme is stable to at least 4 freeze/thaw cycles. To minimize the number of freeze/thaw cycles, aliquot the HDAC2 into separate tubes before storing at -80°C.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

在网站页面上,产品编号会附带包装尺寸/数量一起显示(例如:T1503-25G)。请确保 在“产品编号”字段中仅输入产品编号 (示例: T1503).

示例

T1503
货号
-
25G
包装规格/数量

其它示例:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

遇到问题?欢迎随时联系我们技术服务 寻求帮助

批号可以在产品标签上"批“ (Lot或Batch)字后面找到。

Aldrich 产品

  • 如果您查询到的批号为 TO09019TO 等,请输入去除前两位字母的批号:09019TO。

  • 如果您查询到的批号含有填充代码(例如05427ES-021),请输入去除填充代码-021的批号:05427ES。

  • 如果您查询到的批号含有填充代码(例如 STBB0728K9),请输入去除填充代码K9的批号:STBB0728。

未找到您寻找的产品?

部分情况下,可能未在线提供COA。如果搜索不到COA,可在线索取。

索取COA

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门