所有图片(1)

文件

COA分析证书/COQ

04-1530

抗MDM2抗体，克隆3G9

clone 3G9, from mouse

所有图片(1)

别名:

Double minute 2 protein, Mdm2 p53 binding protein homolog (mouse), Mdm2, transformed 3T3 cell double minute 2, p53 binding protein, Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse), Oncoprotein Mdm2, double minute 2, human homolog

eCl@ss:

32160702

NACRES:

NA.41

比较类似商品

查看完整比较结果

Show Differences

1 of 4

此商品	04-1555	04-1556	MABE340-AF488
Sigma-Aldrich 04-1530 抗MDM2抗体，克隆3G9	Sigma-Aldrich 04-1555 Anti-Mdmx Antibody, clone 8C6	Sigma-Aldrich 04-1556 Anti-MDM4 Antibody, clone 7A8	Sigma-Aldrich MABE340-AF488 Anti-MDM2 Antibody, clone IF2, Alexa Fluor^™ 488 Conjugate
antibody form purified antibody	antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin
clone 3G9, monoclonal	clone 8C6, monoclonal	clone 7A8, monoclonal	clone IF2, monoclonal
species reactivity human	species reactivity human	species reactivity mouse, human	species reactivity human
packaging antibody small pack of 25 μL	packaging -	packaging -	packaging -
technique(s) immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable	technique(s) ChIP: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable	technique(s) western blot: suitable	technique(s) immunocytochemistry: suitable

一般描述

MDM2在许多肿瘤中过表达。它的主要功能是p53抑癌蛋白的泛素化和降解。当Thr-216未被磷酸化时，该单克隆抗体还可识别鼠MDM2的Thr-216周围的肽表位。SMP14还可与某些细胞角蛋白（6、14 & 16）发生交叉反应。这仅在处理某些上皮细胞时会产生此问题，而成纤维细胞不会。

特异性

该抗体可识别MDM2。

免疫原

带His标签的重组蛋白，对应于人MDM2。

表位：未知

应用

免疫沉淀分析：一个代表性批次已在一个独立实验室中进行此应用。(Changgong, L., et al. (2002). Molecular and Cellular Biology.22(21):7562-7571.)

抗MDM2抗体，克隆3G9，是一种小鼠单克隆抗体，可用于检测MDM2（也称为双微2蛋白或Mdm2 p53结合蛋白同源物） &，其已通过WB、IP、ICC、&IHC验证。

研究子类别
RNA代谢 & 结合蛋白

泛素 & 泛素代谢

研究类别
表观遗传学&核功能

质量

通过蛋白质印迹对MCF-7细胞裂解液进行了评估。

蛋白质印迹分析：0.5 µg/ml该抗体可在10 µg的MCF-7细胞裂解液中检测到MDM2。

目标描述

~75 kDa

外形

形式：纯化

纯化的小鼠单克隆IgGκ，溶于含有0.1 M Tris-甘氨酸（pH 7.4，150 mM NaCl）和0.05％叠氮化钠的缓冲液中。

蛋白G纯化

储存及稳定性

自发运之日起，在 2-8°C 条件下可稳定保存1年

分析说明

对照
MCF-7细胞裂解液

其他说明

浓度：请参考批次特异性浓缩物的分析证书。

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

ZRB1127

Anti-MDM2 Antibody, clone Z6H3 ZooMAb^® Rabbit Monoclonal, recombinant, expressed in HEK 293 cells

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

如何查找产品货号

在网站页面上，产品编号会附带包装尺寸/数量一起显示（例如：T1503-25G）。请确保在“产品编号”字段中仅输入产品编号 (示例 T1503).

示例

T1503

货号

25G

包装规格/数量

其它示例：

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

请输入1.000309185)

遇到问题？欢迎随时联系我们技术服务寻求帮助

如何查找COA批号

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich 产品

如果您查询到的批号为 TO09019TO 等，请输入去除前两位字母的批号：09019TO。
如果您查询到的批号含有填充代码（例如05427ES-021），请输入去除填充代码-021的批号：05427ES。
如果您查询到的批号含有填充代码（例如 STBB0728K9），请输入去除填充代码K9的批号：STBB0728。

未找到您寻找的产品？

部分情况下，可能未在线提供COA。如果搜索不到COA，可在线索取。

索取COA

A fluorescent probe for imaging p53-MDM2 protein-protein interaction.

Zhenzhen Liu et al.

Chemical biology & drug design, 85(4), 411-417 (2014-09-18)

In this article, we describe a no-wash small-molecule fluorescent probe for detecting and imaging p53-MDM2 protein-protein interaction based on an environment-sensitive fluorescent turn-on mechanism. After extensive biological examination, this probe L1 exhibited practical activity and selectivity in vitro and in

The Dysfunctional MDM2-p53 Axis in Adipocytes Contributes to Aging-Related Metabolic Complications by Induction of Lipodystrophy.

Zhuohao Liu et al.

Diabetes, 67(11), 2397-2409 (2018-08-23)

Profound loss and senescence of adipose tissues are hallmarks of advanced age, but the underlying cause and their metabolic consequences remain obscure. Proper function of the murine double minute 2 (MDM2)-p53 axis is known to prevent tumorigenesis and several metabolic

The MDM2-p53-pyruvate carboxylase signalling axis couples mitochondrial metabolism to glucose-stimulated insulin secretion in pancreatic β-cells.

Xiaomu Li et al.

Nature communications, 7, 11740-11740 (2016-06-07)

Mitochondrial metabolism is pivotal for glucose-stimulated insulin secretion (GSIS) in pancreatic β-cells. However, little is known about the molecular machinery that controls the homeostasis of intermediary metabolites in mitochondria. Here we show that the activation of p53 in β-cells, by

IER2-induced senescence drives melanoma invasion through osteopontin.

Lenka Kyjacova et al.

Oncogene, 40(47), 6494-6512 (2021-10-07)

Expression of the immediate-early response gene IER2 has been associated with the progression of several types of cancer, but its functional role is poorly understood. We found that increased IER2 expression in human melanoma is associated with shorter overall survival

Snail1-dependent p53 repression regulates expansion and activity of tumour-initiating cells in breast cancer.

Ting Ni et al.

Nature cell biology, 18(11), 1221-1232 (2016-10-28)

The zinc-finger transcription factor Snail1 is inappropriately expressed in breast cancer and associated with poor prognosis. While interrogating human databases, we uncovered marked decreases in relapse-free survival of breast cancer patients expressing high Snail1 levels in tandem with wild-type, but

查看所有相关文献

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门

文件

抗MDM2抗体，克隆3G9

clone 3G9, from mouse

推荐产品

属性

生物来源

质量水平

抗体形式

antibody product type

克隆

species reactivity

species reactivity (predicted by homology)

包装

technique(s)

NCBI登记号

UniProt登记号

运输

Gene Information