04-1530

Sigma-Aldrich

抗MDM2抗体,克隆3G9

clone 3G9, from mouse

别名:
Double minute 2 protein, Mdm2 p53 binding protein homolog (mouse), Mdm2, transformed 3T3 cell double minute 2, p53 binding protein, Mdm2, transformed 3T3 cell double minute 2, p53 binding protein (mouse), Oncoprotein Mdm2, double minute 2, human homolog
eCl@ss:
32160702
NACRES:
NA.41
价格与库存信息目前不能提供

Quality Level

biological source

mouse

antibody product type

primary antibodies

clone

3G9, monoclonal

species reactivity

human

species reactivity (predicted by homology)

mouse (based on 100% sequence homology)

packaging

antibody small pack of 25 μL

application(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

ambient

General description

MDM2在许多肿瘤中过表达。它的主要功能是p53抑癌蛋白的泛素化和降解。当Thr-216未被磷酸化时,该单克隆抗体还可识别鼠MDM2的Thr-216周围的肽表位。SMP14还可与某些细胞角蛋白(6、14 & 16)发生交叉反应。这仅在处理某些上皮细胞时会产生此问题,而成纤维细胞不会。

Specificity

该抗体可识别MDM2。

Immunogen

带His标签的重组蛋白,对应于人MDM2。
表位:未知

Application

免疫沉淀分析: 一个代表性批次已在一个独立实验室中进行此应用。(Changgong, L., et al. (2002). Molecular and Cellular Biology.22(21):7562-7571.)
研究子类别
RNA代谢 & 结合蛋白

泛素 & 泛素代谢
抗MDM2抗体,克隆3G9,是一种小鼠单克隆抗体,可用于检测MDM2(也称为双微2蛋白或Mdm2 p53结合蛋白同源物) &,其已通过WB、IP、ICC、&IHC验证。
研究类别
表观遗传学&核功能

Quality

通过蛋白质印迹对MCF-7细胞裂解液进行了评估。

蛋白质印迹分析:0.5 µg/ml该抗体可在10 µg的MCF-7细胞裂解液中检测到MDM2。

Target description

~75 kDa

Physical form

纯化的小鼠单克隆IgGκ,溶于含有0.1 M Tris-甘氨酸(pH 7.4,150 mM NaCl)和0.05%叠氮化钠的缓冲液中。
形式:纯化
蛋白G纯化

Storage and Stability

自发运之日起,在 2-8°C 条件下可稳定保存1年

Analysis Note

对照
MCF-7细胞裂解液

Other Notes

浓度:请参考批次特异性浓缩物的分析证书。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
Zhuohao Liu et al.
Diabetes, 67(11), 2397-2409 (2018-08-23)
Profound loss and senescence of adipose tissues are hallmarks of advanced age, but the underlying cause and their metabolic consequences remain obscure. Proper function of the murine double minute 2 (MDM2)-p53 axis is known to prevent tumorigenesis and several metabolic...
Xiaomu Li et al.
Nature communications, 7, 11740-11740 (2016-06-07)
Mitochondrial metabolism is pivotal for glucose-stimulated insulin secretion (GSIS) in pancreatic β-cells. However, little is known about the molecular machinery that controls the homeostasis of intermediary metabolites in mitochondria. Here we show that the activation of p53 in β-cells, by...
R C Heijkants et al.
Oncogenesis, 7(3), 33-33 (2018-03-30)
Uveal melanoma (UM) is the most frequent ocular cancer in adults, accounting for ~5% of the total melanoma incidence. Although the primary tumor is well treatable, patients frequently develop metastases for which no curative therapy exists. Highly activated protein kinase...
Yemin Wang et al.
Cancer research, 74(12), 3218-3227 (2014-04-26)
The tumor suppressor p53 and miRNAs are linked through a complex network. Several miRNAs modulate p53 expression, while p53 regulates the transcription and/or biogenesis of several other miRNAs. Here, we report the development of a cell-based assay used with a...
Ting Ni et al.
Nature cell biology, 18(11), 1221-1232 (2016-10-28)
The zinc-finger transcription factor Snail1 is inappropriately expressed in breast cancer and associated with poor prognosis. While interrogating human databases, we uncovered marked decreases in relapse-free survival of breast cancer patients expressing high Snail1 levels in tandem with wild-type, but...

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