06-720

Sigma-Aldrich

抗HDAC1抗体

Upstate®, from rabbit

别名:
histone deacetylase 1, reduced potassium dependency, yeast homolog-like 1
eCl@ss:
32160702
NACRES:
NA.41
价格与库存信息目前不能提供

biological source

rabbit

Quality Level

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, mouse, rat

packaging

antibody small pack of 25 μg

mfr. no.

Upstate®

application(s)

activity assay: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

ambient

General description

哺乳动物HADC1(也称为HD1)、HDAC2(也称为哺乳动物RPD3)和HDAC3,均与酵母转录调节子Rpd3p有关,被鉴定为组蛋白脱乙酰基酶。组蛋白的去乙酰化与转录沉默有关。

Specificity

可识别并特异于HDAC1,分子量65 kDa。可能在〜35 kDa处检测到非特异性蛋白。
与其他物种的交叉反应性还未经测试。

Immunogen

6X-His标记的片段,对应于小鼠组蛋白脱乙酰基酶1(HDAC1)的氨基酸53-482。

Application

使用在EA、ICC、IP、WB中验证的抗HDAC1抗体(兔多克隆抗体)检测HDAC1(也称为组蛋白脱乙酰基酶1)。
免疫沉淀
5 μg先前批次产品已在500 μg 3T3/A31 RIPA裂解液中对HDAC1进行了免疫沉淀。

免疫细胞化学:
据报道,该抗体可在1%低聚甲醛固定、0.1%Triton X-100透化的3T3细胞中对HDAC1进行免疫染色。

HDAC活性:
据报道该抗体可对活性HDAC进行免疫沉淀。
研究类别
表观遗传学&核功能
研究子类别
组蛋白

Quality

已通过蛋白质印迹对3T3/A31细胞的RIPA裂解物进行了评估。

蛋白质印迹分析:
0.5-2 μg/mL该批次产品在3T3/A31细胞的RIPA裂解物中检测到HDAC1。

Target description

65kda

Physical form

纯化的兔单克隆抗体IgG,溶于含有0.1 M Tris-甘氨酸(pH 7.4)、0.15 mM NaCl和0.05%叠氮化钠的缓冲液中。冻存于 -20ºC。
形式:纯化
蛋白 A 色谱

Storage and Stability

自收到之日起,在 -20°C 条下可稳定保存 1 年。

处理建议:
第一次融化后,在取下瓶盖之前,将小瓶离心并轻轻混合溶液。 分装到微量离心管中,并储存于 -20°C。 避免反复冻融循环,以免损坏 IgG 和影响产品性能。

Analysis Note

对照
NIH/3T3细胞提取物
包含的阳性抗原对照:货号12-305,3T3/A31细胞裂解液。 加入2.5 μL 2-巯基乙醇/100 μl裂解液,煮沸5分钟以还原样本。在小型凝胶中每个泳道上样 20 μg 经还原的裂解液。

Other Notes

浓度:请参考批次特异性浓缩物的分析证书。

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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