所有图片(2)

07-146

Sigma-Aldrich

Anti-Histone H2A (acidic patch) Antibody

serum, Upstate®

别名:
H2A histone family, member Q, histone 2, H2ac, histone IIa, histone cluster 2, H2ac
eCl@ss:
32160702
NACRES:
NA.41

生物来源

rabbit

质量水平

100

抗体形式

serum

antibody product type

primary antibodies

克隆

polyclonal

species reactivity

rat, human, chicken, mouse, yeast, Saccharomyces cerevisiae

manufacturer/tradename

Upstate®

应用

ChIP: suitable
western blot: suitable

同位素/亚型

IgG

NCBI登记号

UniProt登记号

运输

dry ice

Gene Information

一般描述

Histone H2A is one of the 5 main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2A is involved with the structure of the nucleosomes of the ′beads on a string′ structure.

特异性

Broad species cross-reactivity is expected.
Recognizes Histone H2A, Mr 14 kDa.

免疫原

KLH-conjugated, synthetic peptide corresponding to amino acids 88-97 (IRNDEELNKL-C) of human Histone H2A.

应用

Anti-Histone H2A (acidic patch) Antibody is a Rabbit Polyclonal Antibody for detection of Histone H2A (acidic patch) also known as histone 2-H2ac, histone IIa & has been validated in ChIP & WB.
Research Sub Category
Histones
Research Category
Epigenetics & Nuclear Function
Immunofluorescence:
Recommend Anti-phospho-Histone H2A.X (Ser139), clone JBW301, catalog #05-636.

质量

Routinely evaluated by western blot on acid extracts from HeLa cell lysate.

Western Blot Analysis:
1:1000-1:5000 dilutions of this lot detected Histone H2A (acidic patch) in acid extracts from HeLa cell lysate.

目标描述

14 kDa

外形

Unpurified
Rabbit antiserum polyclonal IgG in buffer containing 0.05% sodium azide. Frozen solution.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
HeLa cell extract.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

分析证书

原产地证书 (CofO)

The histone H2A variant macroH2A1 does not localize to the centrosome.
Friedman, N; Barzily-Rokni, M; Isaac, S; Eden, A
Testing null
Regulation of the activation of the Fanconi anemia pathway by the p21 cyclin-dependent kinase inhibitor.
Rego MA, Harney JA, Mauro M, Shen M, Howlett NG
Oncogene null
2-Amino-3-methylimidazo[4,5-f]quinoline (IQ) promotes mouse hepatocarcinogenesis by activating transforming growth factor-? and Wnt/?-catenin signaling pathways.
Xie, XL; Wei, M; Kakehashi, A; Yamano, S; Tajiri, M; Wanibuchi, H
Toxicological Sciences null
Active VSG expression sites in Trypanosoma brucei are depleted of nucleosomes.
Stanne TM, Rudenko G
Eukaryotic Cell null
Michael Papetti et al.
American journal of physiology. Gastrointestinal and liver physiology, 301(3), G508-G518 (2011-07-09)
Altered profiles of gene expression reflect the reprogramming of intestinal epithelial cells during their maturation along the crypt-luminal axis. To focus on genes important in this process, and how they in turn are regulated, we identified 14 transcripts commonly downregulated

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