07-164

Sigma-Aldrich

抗磷酸化H2A.X(Ser139)抗体

Upstate®, from rabbit

别名:
H2A histone family, member Q, histone 2, H2ac, histone IIa, histone cluster 2, H2ac
eCl@ss:
32160702
NACRES:
NA.41
价格与库存信息目前不能提供

biological source

rabbit

Quality Level

antibody product type

primary antibodies

clone

polyclonal

species reactivity

human, vertebrates

packaging

antibody small pack of 25 μg

mfr. no.

Upstate®

application(s)

ChIP: suitable (ChIP-seq)
immunoprecipitation (IP): suitable
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

shipped in

ambient

Gene Information

human ... HIST2H2AC(8338)

General description

组蛋白H2A是参与真核细胞染色质结构的5种主要组蛋白之一。H2A具有一个主要的球状结构域和一个长N末端的尾巴,其与核糖体核小体′串珠′结构体的结构有关。

Specificity

在Ser139处磷酸化的组蛋白H2A.X。
基于同源序列的保守型,预测其会发生广泛的物种交叉反应。

Immunogen

表位:aa134-142

Application

免疫沉淀
先前批次的该抗体已由独立实验室成功地使磷酸化的H2A.X免疫沉淀。
抗磷酸化H2A.X(Ser139)抗体是一种兔多克隆抗体,可用于检测磷酸化H2A.X(Ser139)(也被称为组蛋白2 H2ac、组蛋白IIa) & 已在IP&和WB中进行了验证。

Quality

已通过蛋白质印迹对未经处理和经星形孢菌素处理的Jurkat裂解液进行了常规评估。

蛋白质印迹分析:
该批次产品以1:500的稀释度在10 μg星形孢菌素处理的Jurkat裂解液中检测到磷酸化H2A.X。

Target description

15kda

Linkage

替代品:MABE205

Physical form

Protein A chromatography
形式:纯化

Storage and Stability

自收到之日起,在 -20°C 条件下可稳定保存 1 年。
处理建议:收到后,在取下瓶盖之前,将小瓶离心并轻轻混合溶液。分装到微量离心管中,并储存于 -20°C。
避免反复冻融,其可能会破坏IgG并影响产品性能。注意:冷藏室温度变化至低于-20°C时可能导致-含甘油的溶液在储存过程中冻结。

Analysis Note

对照
星形孢菌素处理的Jurkat裂解液、紫外线处理的HT-29细胞、阿霉素处理的HT-29细胞。

Other Notes

浓度:请参考批次特异性浓缩物的分析证书。

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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