Merck
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文件

07-434

Sigma-Aldrich

抗-BRCA1抗体

serum, Upstate®

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别名:
Anti-BRCAI, Anti-BRCC1, Anti-BROVCA1, Anti-FANCS, Anti-IRIS, Anti-PNCA4, Anti-PPP1R53, Anti-PSCP, Anti-RNF53
eCl@ss:
32160702
NACRES:
NA.41

生物来源

rabbit

质量水平

抗体形式

serum

antibody product type

primary antibodies

克隆

polyclonal

species reactivity

human

manufacturer/tradename

Upstate®

technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

同位素/亚型

IgG

NCBI登记号

UniProt登记号

运输

dry ice

target post-translational modification

unmodified

Gene Information

human ... BRCA1(672)

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此商品
AB9986SAB5700188SAB5700340
Anti-BRCA1 Antibody serum, Upstate®

Sigma-Aldrich

07-434

抗-BRCA1抗体

antibody form

serum

antibody form

affinity purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

species reactivity

human

species reactivity

rhesus macaque, horse, human

species reactivity

human, mouse

species reactivity

rat, human, mouse

manufacturer/tradename

Upstate®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

technique(s)

immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

dot blot: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable

technique(s)

immunofluorescence: 1:20-1:50, immunohistochemistry: 1:50-1:200, western blot: 1:500-1:2000

technique(s)

immunofluorescence: 1:50-1:200, immunohistochemistry: 1:50-1:200, western blot: 1:500-1:2000

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一般描述

已经确定了乳腺癌的两个主要易感基因BRCA1和BRCA2。这两个基因都被认为是肿瘤抑制基因。BRCA1和BRCA2内的突变是大多数家族性乳腺癌病例的原因。BRCA1和BRCA2基因产物的功能分析已经确定了它们在转录调控和DNA损伤修复中的双重参与。 BRCA1通过p53和p21基因影响细胞周期调节,并且由于表达降低而导致的BRCA1功能丧失导致细胞周期停滞。在体内,BRCA1和BRCA2在快速增殖的细胞中以最大水平表达。此特征与BRCA1和BRCA2以细胞周期依赖性方式表达的体外观察结果一致。在乳腺发育过程中,BRCA1和BRCA2的表达在快速增殖的细胞区室中被诱导。

特异性

识别人BRCA1。

免疫原

GST标记的融合蛋白对应人BRCA1的1301-1863位氨基酸。

应用

用抗BRCA1抗体(兔多克隆抗体)检测BRCA1,已在IHC、IP & WB中起作用。 这种BRCA1抗体已在人类样本中进行了测试。
研究子类别
细胞周期,DNA 复制&修复
研究类别
表观遗传学&核功能

质量

已通过免疫印迹法对HeLa核提取物进行常规评估

目标描述

220 kDa

外形

含有0.035%叠氮化钠和30%甘油的血清。在-20°C为液体形式。
未纯化

储存及稳定性

自收到之日起在-20°C可稳定保存2年。避免反复冻融。为了最大程度地回收产品,在融化后和取下盖子之前,将原始样品瓶进行离心。

分析说明

对照
阳性抗原对照:货号12-309,HeLa细胞核提取物。加入等体积的Laemmli样品还原性缓冲液至10 μL提取物中,煮沸5分钟以对样品进行还原。在微凝胶每个泳道上样20 μg经还原的提取物。

其他说明

浓度:请参考批次特异性浓缩物的分析证书。

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Ligand-dependent differences in estrogen receptor beta-interacting proteins identified in lung adenocarcinoma cells corresponds to estrogenic responses.
Ivanova, M; Abner, S; Pierce, W; Klinge, C
Proteome Science null
Yifan Wang et al.
The Journal of clinical investigation, 126(8), 3145-3157 (2016-07-28)
Patients with cancers that harbor breast cancer 1 (BRCA1) mutations initially respond well to platinum and poly(ADP-ribose) polymerase inhibitor (PARPi) therapy; however, resistance invariably arises in these patients and is a major clinical problem. The BRCA1185delAG allele is a common
Aurélia Noll et al.
Cancer cell international, 16, 53-53 (2016-07-05)
Poly(ADP-ribose) polymerase (PARP) inhibitors have entered the clinics for their promising anticancer effect as adjuvant in chemo- and radiotherapy and as single agent on BRCA-mutated tumours. Poly(ADP-ribose) glycohydrolase (PARG) deficiency was also shown to potentiate the cytotoxicity of genotoxic agents
BAL1 and its partner E3 ligase, BBAP, link Poly(ADP-ribose) activation, ubiquitylation, and double-strand DNA repair independent of ATM, MDC1, and RNF8.
Yan, Q; Xu, R; Zhu, L; Cheng, X; Wang, Z; Manis, J; Shipp, MA
Molecular and cellular biology null
Qingsheng Yan et al.
Molecular cell, 36(1), 110-120 (2009-10-13)
Although the BBAP E3 ligase and its binding partner BAL are overexpressed in chemotherapy-resistant lymphomas, the role of these proteins in DNA damage responses remains undefined. Because BAL proteins modulate promoter-coupled transcription and contain structural motifs associated with chromatin remodeling

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