Merck
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文件

1.00439

Millipore

ChromoCult® Listeria Agar Enrichment-Supplement

ChromoCult®, selective for Listeria spp.

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别名:
Listeria Enrichment Supplement

Agency

APHA
ISO 11133
ISO 11290-1
ISO 11290-2

质量水平

形式

lyophilized powder

制造商/商品名称

ChromoCult®

技术

microbiological culture: suitable

pH值(酸碱度)

6.6 (20 °C in H2O)

应用

food and beverages
microbiology
pathogen testing

储存温度

2-8°C

适用性

selective for Listeria spp. (Agar)

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1.004271.1178103708
technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

-

form

lyophilized powder

form

medium granules (dehydrated (DCM))

form

solid

form

liquid

application(s)

food and beverages
microbiology
pathogen testing

application(s)

food and beverages
microbiology

application(s)

food and beverages
microbiology

application(s)

environmental
food and beverages, microbiology

storage temp.

2-8°C

storage temp.

15-25°C

storage temp.

2-8°C

storage temp.

−20°C

pH

6.6 (20 °C in H2O)

pH

7.0-7.4 (25 °C, 70 g/L in H2O)

pH

-

pH

-

一般描述

ChromoCult® Listeria Agar Enrichment-Supplement is a homogeneous, sterile suspension of L-α-phosphatidylinositol in distilled water. Differentiation of Listeria monocytogenes from non-pathogenic Listeria sp. is achieved through the production of a phosphatidylinositol-specific phospholipase C (PI-PLC). Listeria monocytogenes hydrolyses the specific purified substrate added to the medium producing an opaque halo around the colonies.

应用

ChromoCult® Listeria Agar Enrichment-Supplement is used as an additive for the preparation of ChromoCult® Listeria Selective Agar for detection and enumeration of Listeria monocytogenes and of Listeria sp. in a variety of food products.

联系

Replaces: 1004390017

分析说明

Growth promotion test according to ISO 11133.
Inoculum on reference medium
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
10 - 100
- Listeria monocytogenes ATCC 19111 (WDCM 00020): 10 - 100
- Listeria innocua ATCC 33090 (WDCM 00017): 10 - 100
Recovery on test medium
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
≥ 70 %
- Listeria monocytogenes ATCC 19111 (WDCM 00020): ≥ 70 %
- Listeria innocua ATCC 33090 (WDCM 00017): ≥ 70 %
Colonies
- Listeria monocytogenes ATCC 13932 (WDCM 00021):
blue-green; non-transparent zone
- Listeria monocytogenes ATCC 19111 (WDCM 00020): blue-green; non-transparent zone
- Listeria innocua ATCC 33090 (WDCM 00017): blue-green
Growth
- Escherichia coli ATCC 25922 (WDCM 00013):
no growth
- Escherichia coli ATCC 8739 (WDCM 00012): no growth
- Enterococcus faecalis ATCC 19433 (WDCM 00009): no growth
- Enterococcus faecalis ATCC 29212 (WDCM 00087): no growth
Incubation: Listeria 24-48 Std. 37°C aerobic; other strains at least 48 hrs.

法律信息

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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商品

Detection of Listeria monocytogenes in food and environmental samples according to ISO 11290-1, FDA-BAM and USDA-FSIS

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