所有图片(1)

113200

Sigma-Aldrich

Anti-α-Actin Mouse mAb (1A4)

liquid, clone 1A4, Calbiochem®

质量水平

100

生物来源

mouse

antibody product type

primary antibodies

克隆

1A4, monoclonal

形式

liquid

包含

≤0.1% sodium azide as preservative

species reactivity

human, rabbit, baboon

manufacturer/tradename

Calbiochem®

储存条件

do not freeze

同位素/亚型

IgG2a

运输

wet ice

储存温度

2-8°C

一般描述

Recognizes α-actin in smooth muscle cells of blood vessels and parenchymal tissue of the intestine, testis, and ovary. Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).
  • Antibody Target Gene Symbol: ACTA2
  • Target Synonym: 0610041G09RIK, a-SMA, AAT6, ACTSA, Actvs, ALPHA ACTIN VSM, ALPHA SMA, ALPHA SMOOTH MUSCLE ACTIN, ALPHA-ACTIN 2, FLJ45296, MGC127800, RGD: 621676, Similar to vascular alpha actin, Sm alpha actin, SMalphaA, SMOOTH MUSCLE ALPHA ACTIN
  • Entrez Gene Name: actin, alpha 2, smooth muscle, aorta
  • Hu Entrez ID: 59 (Related Antibodies: 113200)
  • Rat Entrez ID: 81633
  • Mouse monoclonal antibody. Recognizes α-actin in smooth muscle cells.
    This Anti-α-Actin Mouse mAb (1A4) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of α-Actin.

    免疫原

    Human Smooth Muscle
    a synthetic peptide corresponding to amino acids 1-10 of human smooth muscle α-actin.

    包装

    500 μL in Plastic ampoule
    Please refer to vial label for lot-specific concentration.

    应用

    Frozen Sections (1:50-1:75)

    Immunoblotting (see comments)

    Paraffin Sections (1:50-1:75)

    警告

    Toxicity: Standard Handling (A)

    外形

    In 150 mM NaCl, 20 mM Tris-borate, BHC-6 carrier polysaccharides, pH 7.5.

    分析说明

    Positive Control
    Smooth muscle cells of blood vessels and parenchymal tissue of intestine, testis, or ovary
    Negative Control
    Fibroblasts, striated muscle, or myocardium

    其他说明

    Rannov-Jennsen, L., et al. 1990. Lab Invest.63, 532.
    Skalli, O., et al. 1989. J. Histochem. Cytochem37, 315.
    Skalli, O., et al. 1986. J. Cell. Biol. 103, 2787.
    Does not recognize actin from fibroblasts (β- and γ-cytoplasmic), striated muscle (α-sarcomeric), or myocardium (α-myocardial).Prolonged fixation in buffered formalin destroys the epitope. The antibody is specific for the α-smooth muscle isoform of actin. This antibody is also reported to work for immunoblotting. Variables associated with assay conditions will dictate the proper working dilution.

    法律信息

    CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

    储存分类代码

    12 - Non Combustible Liquids

    WGK

    WGK 2

    闪点(F)

    Not applicable

    闪点(C)

    Not applicable

    分析证书

    原产地证书 (CofO)

    Activation of the NF-I?B pathway alters the phenotype of MSCs in the tracheal aspirates of preterm infants with severe BPD.
    Reicherzer, et al.
    American Journal of Physiology. Lung Cellular and Molecular Physiology, 315, L87-L101 (2020)
    null
    Bridget M Seitz et al.
    American journal of physiology. Heart and circulatory physiology, 313(3), H676-H686 (2017-06-20)
    Serotonin [5-hydroxytryptamine (5-HT)] causes relaxation of the isolated superior mesenteric vein, a splanchnic blood vessel, through activation of the 5-HT7 receptor. As part of studies designed to identify the mechanism(s) through which chronic (≥24 h) infusion of 5-HT lowers blood
    David J Ferland et al.
    Vascular pharmacology, 88, 30-41 (2016-11-29)
    Chemerin is an adipokine associated with increased blood pressure, and may link obesity with hypertension. We tested the hypothesis that chemerin-induced contraction of the vasculature occurs via calcium flux in smooth muscle cells. Isometric contraction of rat aortic rings was
    Christine Chaponnier et al.
    F1000Research, 5, 416-416 (2016-06-24)
    Higher vertebrates (mammals and birds) express six different highly conserved actin isoforms that can be classified in three subgroups: 1) sarcomeric actins, α-skeletal (α-SKA) and α-cardiac (α-CAA), 2) smooth muscle actins (SMAs), α-SMA and γ-SMA, and 3) cytoplasmic actins (CYAs)

    我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

    联系技术服务部门