Merck
All Photos(1)

1.15444

Sigma-Aldrich

考马斯亮蓝G250 (C.I.42655)

for electrophoresis

Synonym(s):
Coomassie亮蓝 G 250 (C.I.42655), SERVA蓝G, 极地蓝G, 酸性蓝90, 青蓝G
Empirical Formula (Hill Notation):
C47H48N3NaO7S2
CAS Number:
Molecular Weight:
854.02
MDL编号:
EC索引号:
228-058-4

质量水平

效能

>5000 mg/kg LD50, oral (Rat)

technique(s)

protein staining: suitable

pH值(酸碱度)

6.4 (20 °C, 10 g/L in H2O)

溶解性

40 g/L

堆积密度

520 kg/m3

储存温度

no temp limit

InChI

1S/C47H49N3O7S2.Na/c1-6-49(31-35-11-9-13-43(29-35)58(51,52)53)40-21-25-45(33(4)27-40)47(37-15-17-38(18-16-37)48-39-19-23-42(24-20-39)57-8-3)46-26-22-41(28-34(46)5)50(7-2)32-36-12-10-14-44(30-36)59(54,55)56;/h9-30,48H,6-8,31-32H2,1-5H3,(H-,51,52,53,54,55,56);/q;+1/p-1

InChI key

CMGWKYXJIYYESN-UHFFFAOYSA-M

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1.125531.041771.01310
technique(s)

protein staining: suitable

technique(s)

protein staining: suitable

technique(s)

-

technique(s)

-

pH

6.4 (20 °C, 10 g/L in H2O)

pH

6.2 (25 °C, 10 g/L in H2O)

pH

2.9 (25 °C, 10 g/L in H2O)

pH

2.5 (25 °C, 10 g/L in H2O)

solubility

40 g/L

solubility

20 g/L

solubility

200 g/L

solubility

100 g/L

storage temp.

no temp limit

storage temp.

2-30°C

storage temp.

2-30°C

storage temp.

2-30°C

Quality Level

100

Quality Level

100

Quality Level

200

Quality Level

200

potency

>5000 mg/kg LD50, oral (Rat)

potency

>5000 mg/kg LD50, oral (Rat)

potency

>2000 mg/kg LD50, oral (Rat)

potency

300-400 mg/kg LD50, oral (Rat)

一般描述

Coomassie亮蓝G 250 (C.I.42655)是Coomassie亮蓝(CBB)之一,是一种三苯基甲烷染料。CBB染料广泛用于十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分离的蛋白质显色。CBB G-250(绿蓝色)染料适用于Bradford测定的蛋白定量。

应用

Coomassie亮蓝G 250 (C.I.42655)已用于十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中的凝胶染色。它还用于Bradford蛋白测定。

分析说明

鉴定标准(UV/VIS-光谱):通过测试
最大吸光值λ最大(缓冲液pH7.0):577 - 584 nm
光谱吸光性A 1%/1cm(λ最大;0.01 g/l;缓冲液pH 7.0;基于干物质计算):450 - 570
TLC-测试:通过测试
干燥损失(100 °C): ≤ 8 %
电泳适用性:通过测试

法律信息

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable


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