Merck
所有图片(1)

1.24568

Sigma-Aldrich

蛋白酶 K

(from Tritirachium album) chromatographically purified lyophilized 30 mAnson-U/mg for biochemistry and molecular biology EC 3.4.21.14

登录to View Organizational & Contract Pricing

Select a Size

别名:
蛋白酶 K 来源于林伯氏白色念球菌, 旋毛虫属碱性蛋白酶, 肽链内切酶
CAS号:
MDL编号:
EC索引号:
254-457-8

质量水平

形式

solid

分子量

28.93 kDa

pH值(酸碱度)

6.2-6.8 (20 °C, 10 g/L in H2O)

密度

1.1 g/cm3

堆积密度

35 kg/m3

储存温度

2-8°C

正在寻找类似产品? Visit 产品对比指南

比较类似商品

查看完整比较结果

显示差异

1 of 4

此商品
P5568P4850SRE0047
Sigma-Aldrich

Sigma-Aldrich

1.24568

蛋白酶 K

mol wt

28.93 kDa

mol wt

28.93 kDa

mol wt

28.93 kDa

mol wt

28.93 kDa

pH

6.2-6.8 (20 °C, 10 g/L in H2O)

pH

-

pH

-

pH

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

400

Quality Level

200

Quality Level

200

Quality Level

400

density

1.1 g/cm3

density

-

density

-

density

-

应用

在分离DNA和RNA的过程中,用于核酸酶的蛋白水解失活。
用于去除内毒素结合的阳离子蛋白,如溶菌酶和核糖核酸酶A。
据报道,其可用于分离肝、酵母和绿豆线粒体
用于测定酶在细胞膜上的定位
用于石蜡包埋组织切片的处理,暴露用于抗体标记的抗原结合位点。
用于可传播海绵状脑病(TSE)研究中朊病毒脑组织样本蛋白的消化。

生化/生理作用

蛋白酶K是一种稳定且具有高活性的丝氨酸蛋白酶。晶体和分子结构研究证据表明该酶属于枯草素家族,具有活性位点催化三联体(Asp39-His69-Ser224)。它在一系列广泛的环境中均稳定:pH,缓冲盐,去污剂(SDS)以及温度。 在0.1-0.5% SDS存在的情况下,蛋白酶K可保持活性,在不损害分离DNA完整性的前提下,可以消化DNA制剂中的多种蛋白质和核酸酶。

分析说明

外观(颜色):白色
外观(描述):冻干粉末
活性(血红蛋白; pH 7.5;37 °C): ≥ 30.0 mAnsonU/mg
比活力(以蛋白质计算): ≥ 40 mAnsonU/mg
脱氧核糖核酸酶(切口活性;pBR 322;6 H;37 °C):未检出
RNA酶(RNA;2 h;37°C):未检出

象形图

Health hazard

警示用语:

Danger

危险声明

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2

储存分类代码

11 - Combustible Solids

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

在网站页面上,产品编号会附带包装尺寸/数量一起显示(例如:T1503-25G)。请确保 在“产品编号”字段中仅输入产品编号 (示例: T1503).

示例

T1503
货号
-
25G
包装规格/数量

其它示例:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

遇到问题?欢迎随时联系我们技术服务 寻求帮助

批号可以在产品标签上"批“ (Lot或Batch)字后面找到。

Aldrich 产品

  • 如果您查询到的批号为 TO09019TO 等,请输入去除前两位字母的批号:09019TO。

  • 如果您查询到的批号含有填充代码(例如05427ES-021),请输入去除填充代码-021的批号:05427ES。

  • 如果您查询到的批号含有填充代码(例如 STBB0728K9),请输入去除填充代码K9的批号:STBB0728。

未找到您寻找的产品?

部分情况下,可能未在线提供COA。如果搜索不到COA,可在线索取。

索取COA

L Bruno et al.
Immunity, 5(4), 343-352 (1996-10-01)
In peripheral lymphoid tissues of TCR transgenic mice that express the nominal antigen (HY peptide plus H-2Db MHC) recognized by the transgenic TCR, there exist unusual CD4-CD8- and CD4-CD8low cells bearing the transgenic TCR. Here we show that, unlike TCR
Takaharu Sasaki et al.
Cell reports, 28(1), 202-217 (2019-07-04)
Complex interactions between immune cells are an important component in the induction of obesity. Here, we show that Il2rg-/-Rag2-/- mice lacking all lymphocytes are resistant to diet-induced obesity. Transplantation of bone marrow cells from Rag2-/- mice, which lack only acquired
Jan P M Langeveld et al.
Veterinary research, 52(1), 59-59 (2021-04-18)
The unconventional infectious agents of transmissible spongiform encephalopathies (TSEs) are prions. Their infectivity co-appears with PrPSc, aberrant depositions of the host's cellular prion protein (PrPC). Successive heat treatment in the presence of detergent and proteolysis by a keratinase from Bacillus
D L Baggesen et al.
Journal of clinical microbiology, 35(1), 330-333 (1997-01-01)
Studies of pairs of Salmonella enterica serovar Enteritidis isolates from different poultry flocks showed that phage type (PT) 7 may be derived from PT 1, 4, and 6. The conversion appeared to be associated with loss of lipopolysaccharide. It is
Julia Bartuli et al.
STAR protocols, 3(1), 101116-101116 (2022-02-05)
The functional and structural characterization of macromolecular complexes requires protocols for their native isolation. Here, we describe a protocol for this task based on the recombinant poxvirus Vaccinia expressing tagged proteins of interest in infected cells. Tagged proteins and their

商品

How Proteinase K Can Be Used in Tissue Samples

The use of Pro K in combination with other reagents, such as detergents and chaotropic agents, can help to disrupt the cell membranes and release DNA from tissue. This is particularly important for downstream applications such as PCR, sequencing, and other molecular biology techniques that require pure and intact DNA.

An Introduction to Proteinase K and Its Applications

Proteinase K is commonly used in molecular biology and biochemistry applications to digest structural proteins and enzymes. It is useful in removing nucleases that can degrade DNA and RNA, as well as in the isolation of intact genomic DNA from various sources.

How Proteinase K Can Be Used in Blood DNA Extraction Applications

In blood DNA extraction, Proteinase K, an enzyme commonly used to degrade proteins, can help break down the cellular and nuclear membranes, releasing DNA from the cells that protect it from degradation and increase purity/yield making it more suitable for various molecular biology techniques.

How to Determine the Best Product & Supplier for Proteinase K

The cost of proteinase K can vary depending on the source, purity, manufacturing process and vendor’s quality management system. It is important to balance the cost with the desired quality, performance, documentation and technical/quality support to select the optimal Proteinase K for the intended application

查看所有结果

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门