Tryptic Soy Agar is a complex nutrient rich medium designed to isolate a wide range of bacteria, yeast and molds in clean rooms and isolators. The medium is supplemented with pyruvate, which efficiently neutralizes the presence of hydrogen peroxide used for decontamination of isolators. The neutralizers lecithin, polysorbate Tween®80, histidine, and sodium thiosulfate inactivate possible residues of active agents of disinfectants. In addition, the plate is supplied with a lockable lid (ICRplus;+) for safe transport after sampling without the risk of losing the lid. ICRplus plates are suitable for aerobic and anaerobic incubation by turning the lid into the close position for aerobic incubation and in the vent position for anaerobic incubation. The plates are triple bagged, and gamma irradiated in the final packaging and used for active and passive air monitoring, as well for personnel monitoring.