1.50454

SeQuant^® ZIC-HILIC^™ 5µm,200Å 150 x 2.1 mm

PEEK-lined stainless steel column, suitable for HPLC

Properties

• 物料: PEEK-lined stainless steel column
• 质量水平: 100
• Agency: suitable for USP L114
• 产品线: SeQuant^®
• 特点: endcapped: no
• 参数: 550 bar pressure; 70 °C max. temp.
• technique(s): HPLC: suitable; LC/MS: suitable; UHPLC-MS: suitable; UHPLC: suitable; mass spectrometry (MS): suitable (High)
• 色谱柱长度 × 内径: 15 cm × 2.1 mm
• 基质: fully porous particle
• 基质活性基团: ZIC-HILIC bonding phase
• 粒径: 5 μm
• 孔径: 200 Å pore size
• operating pH: 2-7.5
• 分离技术: hydrophilic interaction (HILIC)
• 储存温度: no temp limit

Description

一般描述

SeQuant^® ZIC^®-HILIC Columns are designed to retain and separate all types of polar and hydrophilic compounds and for robust chromatography with high selectivity and reproducibility. The silica-based ZIC^®-HILIC sorbent has a bonded stationary phase consisting of a highly polar, permanent zwitterion. Separation selectivity is favored by the 1:1 zwitterion charge balance, which makes the ZIC^®-HILIC Column overall neutral, with weak, but important, ionic interactions. Tuning of the selectivity on the ZIC^®-HILIC Column during method development is facilitated by the pH-inpendent permanent zwitterion, ensuring that only the analytes (and not the column) are affected during eluent optimization.The Pore size of 200 Å makes this column perfectly suitable for the separation of larger polar molecules under HILIC conditions.The column dimension of 250 mm length x 2.1 mm ID is the optimal choice for the separation of complex samples using a UHPLC or optimized HPLC Instrument providing low dwell volumes.Thanks to their favorable retention and robust performance, SeQuant^® HILIC columns have become extremely popular in numerous laboratories around the world. For example, US FDA (United States Food and Drug Administration) has recommended SeQuant^® ZIC^®-HILIC for analysis of melamine and related substances. SeQuant^® HILIC columns excel in stability and low bleed. These features make them particularly suitable for LC-MS applications where high background can lead to signal suppression and interference with quantitative measurements while also increasing instrument wear. Their high stability makes these high-performance HILIC columns also attractive for traditional HPLC applications.Learn more about the Supelco HPLC Column portfolio and Method development:Brochure: HPLC and UHPLC Column Selection Guide Brochure: A Practical Guide to HPLC - Method Development Guide

分析说明

理论塔板数（N/m）（胞嘧啶）： ≥ 60000
对称性（胞嘧啶）：0.95 - 1.40

法律信息

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany

ZIC is a registered trademark of Merck KGaA, Darmstadt, Germany

ZIC-HILIC is a trademark of Merck KGaA, Darmstadt, Germany