Merck
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15-115

Sigma-Aldrich

AMPK Pathway Explorer Antibody MiniPack

1 mg/mL, Upstate®

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别名:
AMP-Activated Protein Kinase
eCl@ss:
32161000
NACRES:
NA.41

生物来源

rabbit

质量水平

抗体形式

purified antibody

antibody product type

primary antibodies

克隆

polyclonal

species reactivity

rat (07-350SP, 07-363SP, 07-681SP), mouse (07-350SP), human (07-350SP, 07-363SP, 07-681SP)

manufacturer/tradename

Upstate®

浓度

1 mg/mL

technique(s)

activity assay: suitable (kinase and phosphatase)
western blot: suitable

NCBI登记号

UniProt登记号

运输

dry ice

Gene Information

human ... PRKAA1(5562)
mouse ... Hsf3(245525)

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此商品
15-11615-10507-626
antibody form

purified antibody

antibody form

-

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

polyclonal

clone

10H11.E12, monoclonal, 7, monoclonal, AM9, monoclonal

clone

-

clone

polyclonal

species reactivity

rat (07-350SP, 07-363SP, 07-681SP), mouse (07-350SP), human (07-350SP, 07-363SP, 07-681SP)

species reactivity

human (05-513SP,05-649SP, 05-740SP), mouse (05-649SP and 05-740SP), rat

species reactivity

-

species reactivity

human

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

concentration

1 mg/mL

concentration

-

concentration

-

concentration

-

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一般描述

Pathway Explorer Antibody MiniPack:
Each Pathway Explorer Antibody Minipack contains three related antibodies as part of a signaling cascade or a combination of total and phosphorylated forms of key signaling targets. Each of the three antibodies are 30% the original pack size. Full size versions of each of the Pathway Explorer antibodies are available for sale individually under the same catalog number with the removal of “SP” off of each one (e.g. 05-591SP can be ordered as 05-591).

AMPK:
AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostasis, and a key target for treating Type 2 diabetes and obesity. AMPK exists as a heterotrimeric complex comprised of catalytic alpha subunits (62 kDa) and non-catalytic beta and gamma subunits. Alpha subunit has at least two isoforms (alpha 1 and alpha 2) which differ in their subcellular localization and AMP-dependence. AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation. Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression. Phosphorylation of threonine 172 in the activation loop of the alpha subunit is a key determinant of AMPK activity.

*See full size versions for corresponding references.

特异性

07-681SP: AMPKα phosphorylated on Thr172.

07-363SP: AMP-Activated Protein Kinase alpha 2 (AMPKα2).

07-350SP: AMPKα1
07-681SP: Expected to cross-react with mouse based on 100% sequence homology.
Cross Reactivty
Varies. See individual Data Sheets

免疫原

Epitope: 07-350SP and 07-363SP: Internal

应用

Research Category
Signaling

Apoptosis & Cancer
Research Sub Category
Insulin/Energy Signaling
This Antibody pack contains Anti-AMPK α1 Antibody, Anti-AMPKα2 Antibody, Anti-phospho-AMPKα Antibody(Thr172).

包装

Each vial is 30% the size of the parent catalog number

组分

07-350SP Anti-AMPK α1; 60 µg

07-363SP Anti-AMPKα2; 60 µg

07-681SP Anti-phospho-AMPKα (Thr172); 60 µg

质量

07-681SP:Routinely evaluated by immunoblot on RIPA lysates from overnight starved HEK293 cells and partially purified rat liver AMPK.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected phospho-AMPK α in RIPA lysates from overnight serum starved HEK293 cells and partially purified rat liver AMPK. Signal was reduced after treatment of lysate with λ-phosphatase.

07-363SP: Routinely evaluated by immunoblot in a rat liver preparation.

Western Blot Analysis:
1-2 μg/mL of this lot detected AMPK α2 in a rat liver preparation.


07-350SP: Routinely evaluated by immunoblot in RIPA lysates from non-stimulated A431 cells.

Western Blot Analysis:
0.5-2 μg/mL of this lot detected AMPK α1 in RIPA lysates from non-stimulated A431 cells.

目标描述

~63 kDa;

外形

Protein A purified
3 individual tubes containing either Anti-AMPK α1; Anti-AMPKα2; or Anti-phospho-AMPKα (Thr172)
Format: Purified

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

分析说明

Control
For 07-681SP use HEK293 cells or rat liver AMPK.

For 07-363SP use Rat liver preparation.

For 07-350SP use A431cell lysate, or lysate of HeLa cells treated with heat shock.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

10 - Combustible liquids

WGK

WGK 2


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Metabolism: clinical and experimental, 99, 67-80 (2019-07-23)
Non-alcoholic fatty liver disease (NAFLD) is the hepatic manifestation of metabolic syndrome. Steroid hormones and bile acids are potent regulators of hepatic carbohydrate and lipid metabolism. Steroid 5β-reductase (AKR1D1) is highly expressed in human liver where it inactivates steroid hormones
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Stroke is the second most common cause of death worldwide. A systematic description and characterization of the strokes and the effects induced in the hippocampus have not been performed so far. Here, we analysed the protein expression in the hippocampus
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A major problem of aging is the disruption of metabolic homeostasis. This is particularly relevant in the brain where it provokes neurodegeneration. Caloric restriction is a physiologic intervention known to delay the deleterious consequences of aging in several species ranging

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