所有图片(3)

17-500M

Millipore

Catch and Release® v2.0 Reversible Immunoprecipitation System

Catch & Release v2.0 Reversible Immunoprecipitation System to purify both native & denatured proteins using a variety of antibodies & sample types. Comes in a package of 5 immunoprecipitations.

eCl@ss:
32161000
NACRES:
NB.22

质量水平

100

species reactivity (predicted by homology)

all

manufacturer/tradename

Catch And Release®
Upstate®

应用

activity assay: suitable (IP-kinase)
immunoprecipitation (IP): suitable
western blot: suitable

运输

wet ice

一般描述

Features & Benefits:
Reproducible: eliminates aspiration steps that typically lead to diminished/lost sample
Convenient: spin-column format dramatically simplifies sample handling
Reversible: simple elution of antigen:antibody complex in either native or denatured form



Use:
This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP′s, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been tested successfully with rabbit, mouse, sheep and goat antibodies. IP using human IgG1-4 should be suitable. IP using chicken antibodies or human IgA, IgD, IgE or IgM is not recommended with this kit. Please read the enclosed product manual before use.

应用

Catch and Release v2.0 should not be used with proteins containing a 6-His tag.

包装

50 immunoprecipitations

组分

Catch and Release Wash Buffer, 10X (Cat.# 20-210)

Catch and Release Non-denaturing Elution Buffer, 4X (Cat.# 20-209)

Catch and Release Denaturing Elution Buffer, 1X (Cat.# 20-284)

20-285

20-216

Catch and Release Capture Tubes

质量

routinely evaluated by immunoprecipitation.

储存及稳定性

All components to be stored at 4°C except the Catch and Release Capture Tubes which are stored at room temperature. Components are stable for 6 months from date of shipment.

法律信息

CATCH AND RELEASE is a registered trademark of Merck KGaA, Darmstadt, Germany
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

分析证书

原产地证书 (CofO)

Wanxia He et al.
Journal of virology, 77(19), 10566-10574 (2003-09-13)
Addition of human papillomavirus (HPV) E7 CDK2/cyclin A or CDK2/cyclin E, purified from either insect cells or bacteria, dramatically upregulates histone H1 kinase activity. Activation is substrate specific, with a smaller effect noted for retinoblastoma protein (Rb). The CDK2 stimulatory...
Hypercholesterolemia-induced Abeta accumulation in rabbit brain is associated with alteration in IGF-1 signaling.
Sharma, Sunita, et al.
Neurobiology of Disease, 32, 426-432 (2008)
E-Y Huang et al.
Cell death & disease, 3, e251-e251 (2012-01-13)
Galectin-1 is a lectin recognized by galactoside-containing glycoproteins, and is involved in cancer progression and metastasis. The role of galectin-1 in radiosensitivity has not previously been investigated. Therefore, this study tests whether galectin-1 is involved in the radiosensitivity mediated by...
Enhanced protein recovery and reproducibility from pull-down assays and immunoprecipitations using spin columns.
A Brymora et al.
Analytical biochemistry, 295(1), 119-122 (2001-07-31)
Activation of FAK is necessary for the osteogenic differentiation of human mesenchymal stem cells on laminin-5.
Roman M Salasznyk,Robert F Klees,Adele Boskey,George E Plopper
Journal of Cellular Biochemistry null

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