Merck
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文件

23-021-M

Sigma-Aldrich

UBE1 Protein, active, 10 µg

Active, full length, recombinant human UBE1. N-terminal 6His-tagged. For use in Enzyme Assays. Functions as an E1 enzyme in ubiquitination assays.

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别名:
Ubiquitin activating enzyme E1, Ubiquitin-like modifier-activating enzyme 1, UBA1, A1S9 Protein, UBE1
eCl@ss:
32160405
NACRES:
NA.41

生物来源

human

质量水平

重组

expressed in baculovirus infected Sf21 cells

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable

NCBI登记号

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UBE1 Protein, active, 10 µg

vibrant-m

23-022-M

UbcH3 Protein, active, 10 µg

vibrant-m

23-051-M

UbcH13/Uev1A Protein, active, 10 µg

vibrant-m

23-036-M

UbcH6 Protein, active, 10 µg

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in E. coli

recombinant

-

technique(s)

activity assay: suitable

technique(s)

activity assay: suitable

technique(s)

activity assay: suitable

technique(s)

activity assay: suitable

Quality Level

100

Quality Level

100

Quality Level

100, 300

Quality Level

100, 300

NCBI accession no.

NM_003334

NCBI accession no.

NM_004359

NCBI accession no.

NM_001032288, NM_003348

NCBI accession no.

NM_003341

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

manufacturer/tradename

Upstate®

一般描述

N-terminal GST-tagged, recombinant human XIAP full length, expressed by baculovirus in Sf21 insect cells

应用

Ubiquitination Cascade Comoponent: E1

生化/生理作用

Ubiquitin activating enzyme E1 (UBE1) catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation. It is responsible for the primary ubiquitin-protein isopeptide bond formation and is a critical component for the initiation of in vitro conjugation reactions. UBE1 activates ubiquitin by the ATP dependent adenylation of its C-terminal glycine carboxyl group. This ubiquitin residue is then linked to the sulphydryl side chain moiety of a cysteine residue within UBE1 by forming a high-energy thiol ester bond, liberating free AMP. There are two active sites within the UBE1 molecule allowing it to accommodate two ubiquitin molecules at one time, with a new ubiquitin forming an adenylate intermediate as the previous one is transferred to the thiol site. The activated ubiquitin is then transferred to the lysine of target proteins via the E2/E3 conjugation cascade.

外形

Purified using immobilized metal affinity chromatography.

其他说明

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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