Merck
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23-044-M

Sigma-Aldrich

VHL Protein Complex, Active, 10 µg

Active complex of five (5) recombinant human enzymes: VHL amino acids 54-end, full length Elongin C, full length Elongin B, full length Cul2, & full length Rbx1. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.

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别名:
VCB-Cul2 complex, ECV complex, CBC VHL
eCl@ss:
32160405
NACRES:
NA.41

生物来源

human

质量水平

重组

expressed in Sf21 cells

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable

Gene Information

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23-023-M23-041-M23-030-M
recombinant

expressed in Sf21 cells

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in baculovirus infected Sf21 cells

recombinant

expressed in baculovirus infected Sf21 cells

technique(s)

activity assay: suitable

technique(s)

activity assay: suitable

technique(s)

activity assay: suitable

technique(s)

activity assay: suitable

NCBI accession no.

NM_000551, NM_003591, NM_005648 (Elongin C), NM_007108 (Elongin B), NM_014248

NCBI accession no.

NM_001826, NM_003592, NM_005983, NM_014248, NM_170679

NCBI accession no.

NM_005188

NCBI accession no.

NM_003592, NM_014248, NM_033632, NM_170679

Quality Level

100

Quality Level

100

Quality Level

100, 300

Quality Level

100

Gene Information

human ... Cul2(8453), Rbx1(9978), VHL(7428)

Gene Information

human ... Cks1(1163), Cul1(8454), Rbx1(9978), Skp1(6500), Skp2(6502)

Gene Information

-

Gene Information

human ... Cul1(8454), Fbw7(55294), Rbx1(9978), Skp1(6500)

一般描述

The VHL complex is a multi-subunit ubiquitin ligase composed of VHL, Elongin B, Elongin C, Cul2 and Rbx1. The VHL protein serves as the substrate recognition component and is linked by Elongin C to a heterodimeric Cul2/Rbx1 module that functions as a potent activator of the ubiquitination of target proteins by an E2 conjugating enzyme. Elongin B interacts with the complex through Elongin C and appears to stabilize the binding of Elongin C to VHL. The primary function of the VHL complex is to regulate HIF (hypoxia inducible factor) activity by targeting the hydroxylated HIF-1α subunit for ubiquitination and rapid proteasomal degradation under normoxic conditions. It therefore plays an important role in the regulation of hypoxia-inducible genes such as the vascular endothelial growth factor (VEGF) and glucose transport-1 (Glut-1). Mutations in VHL are associated with the inherited von Hippel-Lindau (VHL) cancer syndrome and numerous forms of renal cell carcinoma.

应用

Ubiquitination Cascade Comoponent: E3

外形

Purified using glutathione sepharose.

其他说明

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Sang Bae Lee et al.
Molecular cell, 79(3), 376-389 (2020-07-09)
Activation of dual-specificity tyrosine-phosphorylation-regulated kinases 1A and 1B (DYRK1A and DYRK1B) requires prolyl hydroxylation by PHD1 prolyl hydroxylase. Prolyl hydroxylation of DYRK1 initiates a cascade of events leading to the release of molecular constraints on von Hippel-Lindau (VHL) ubiquitin ligase

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