Merck
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268298

Sigma-Aldrich

DAPI,二盐酸盐-CAS 28718-90-3-Calbiochem

Cell-permeable DNA-binding dye.

Synonym(s):
DAPI,二盐酸盐-CAS 28718-90-3-Calbiochem, 4ʹ,6-二脒基-2-苯基吲哚二盐酸盐
Empirical Formula (Hill Notation):
C16H15N5 · 2HCl
CAS Number:
Molecular Weight:
350.25
MDL编号:

质量水平

100

测定

≥90% (HPLC)

形式

solid

manufacturer/tradename

Calbiochem®

储存条件

OK to freeze
desiccated (hygroscopic)
protect from light

颜色

yellow

溶解性

water: 5 mg/mL
ethanol: soluble
methanol: soluble

运输

ambient

储存温度

−20°C

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

InChI key

FPNZBYLXNYPRLR-UHFFFAOYSA-N

一般描述

DNA特异性荧光染料。优先结合富含腺嘌呤和胸苷的DNA(最大激发=359 nm,最大发射=461 nm)。可用于凋亡细胞中细胞核和核DNA的显微镜分析。可用于检测支原体。S-腺苷-L-蛋氨酸脱羧酶和二胺氧化酶的可逆抑制剂。细胞通常以1 µg/ml染色。
细胞可渗透的DNA-结合染料。优先与富含腺嘌呤和胸腺嘧啶的DNA结合。在流式细胞仪中用于测量核DNA含量或对分离的染色体进行分选。可用于正常和凋亡细胞中细胞核和核DNA的显微镜检测。可用于检测支原体。S-腺苷-L-蛋氨酸脱羧酶和二胺氧化酶的可逆抑制剂。也可以5 mg/ml mM的H2O溶液(目录号508741)提供。

包装

10 mg in Plastic ampoule

生化/生理作用

主要靶标
优先与富含腺嘌呤和胸腺嘧啶的DNA结合
产物不与ATP竞争。
可逆性:是
细胞渗透性:否

警告

毒性:标准处理(A)

重悬

复溶后,等分并冷冻长期保存(-20°C),或冷藏(4°C)短期保存。储备溶液在4°C下可稳定保存6周,在-20°C下可稳定保存6个月。

其他说明

Mathieu, C., et al. 1995.Exp.Cell Res.216, 13.
Cubria, C., et al. 1994.Comp.Biochem.Physiol.C. 105, 251.
Hotz, M.A., et al. 1994.Cytometry15, 237.
Lee, L.S., and Garnett, H.M.1993.J. Biochem.Biophys.Methods 26, 249.
Miller, T., et al. 1993.Biotechniques15, 1042.
Gray, J.W., et al. 1982.Science238, 323.
Hessling, J.J., et al. 1980.J. Immunol. Methods38, 315.
Russels, W.C., et al. 1975.Nature253, 461.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

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