Merck
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文件

268298

Sigma-Aldrich

DAPI,二盐酸盐

Cell-permeable DNA-binding dye.

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别名:
DAPI,二盐酸盐, 4ʹ,6-二脒基-2-苯基吲哚二盐酸盐
经验公式(希尔记法):
C16H15N5 · 2HCl
CAS号:
分子量:
350.25
MDL编号:

质量水平

检测方案

≥90% (HPLC)

形式

solid

制造商/商品名称

Calbiochem®

储存条件

OK to freeze
desiccated (hygroscopic)
protect from light

颜色

yellow

溶解性

water: 5 mg/mL
ethanol: soluble
methanol: soluble

荧光

λex ~359 nm
λem ~461 nm

运输

ambient

储存温度

−20°C

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

InChI key

FPNZBYLXNYPRLR-UHFFFAOYSA-N

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此商品
MBD0015MBD0020D9542
DAPI,二盐酸盐 Cell-permeable DNA-binding dye.

268298

DAPI,二盐酸盐

DAPI成品溶液 For Nuclear counterstain in immunoflourecence microscopy, High Content Screening (HCS), Chromosome staining and flow cytometry (FACS)., 1 mg/mL

MBD0015

DAPI成品溶液

DAPI成品溶液,带抗衰减液 For Nuclear counterstain in Fluorescence Microscopy

MBD0020

DAPI成品溶液,带抗衰减液

DAPI for nucleic acid staining

D9542

DAPI

fluorescence

λex ~359 nm, λem ~461 nm

fluorescence

λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

fluorescence

λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

fluorescence

λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)

form

solid

form

liquid

form

liquid

form

powder

solubility

water: 5 mg/mL, methanol: soluble

solubility

water: soluble

solubility

-

solubility

H2O: 20 mg/mL (heat or sonication may be required. Solutions stored in the dark at room temperature or 4 °C should be stable for 2 to 3 weeks.), PBS: insoluble

assay

≥90% (HPLC)

assay

-

assay

-

assay

≥98% (HPLC)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

一般描述

DNA特异性荧光染料。优先结合富含腺嘌呤和胸苷的DNA(最大激发=359 nm,最大发射=461 nm)。可用于凋亡细胞中细胞核和核DNA的显微镜分析。可用于检测支原体。S-腺苷-L-蛋氨酸脱羧酶和二胺氧化酶的可逆抑制剂。细胞通常以1 µg/ml染色。
细胞可渗透的DNA-结合染料。优先与富含腺嘌呤和胸腺嘧啶的DNA结合。在流式细胞仪中用于测量核DNA含量或对分离的染色体进行分选。可用于正常和凋亡细胞中细胞核和核DNA的显微镜检测。可用于检测支原体。S-腺苷-L-蛋氨酸脱羧酶和二胺氧化酶的可逆抑制剂。也可以5 mg/ml mM的H2O溶液(目录号508741)提供。

生化/生理作用

主要靶标
优先与富含腺嘌呤和胸腺嘧啶的DNA结合
产物不与ATP竞争。
可逆性:是
细胞渗透性:否

警告

毒性:标准处理(A)

重悬

复溶后,等分并冷冻长期保存(-20°C),或冷藏(4°C)短期保存。储备溶液在4°C下可稳定保存6周,在-20°C下可稳定保存6个月。

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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