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324682

Sigma-Aldrich

Elastase, Porcine Pancreas, High Purity, Crystallized

Elastase, Porcine Pancreas, High Purity, Crystallized, CAS 39445-21-1, is a serine protease that hydrolyzes proteins and peptides, especially at bonds adjacent to neutral amino acid residues.

别名:
Elastase, Porcine Pancreas, High Purity, Crystallized
CAS号:
MDL编号:

质量水平

100

形式

lyophilized

specific activity

≥25 units/mg protein

manufacturer/tradename

Calbiochem®

储存条件

OK to freeze
desiccated

溶解性

distilled water: 10 mg/mL

异质活性

Chymotrypsin ≤0.2%

运输

ambient

储存温度

−20°C

一般描述

Native elastase from porcine pancreas. Catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin, and of a number of synthetic substrates containing aspartic acid, glutamic acid, phenylalanine, or tyrosine. Preferentially cleaves peptide bonds at the carbonyl end of amino acid residues with small hydrophobic side chains, such as glycine, valine, leucine, isoleucine, and particularly alanine. Inhibited by DFP, elastinal, and α2-macroglobulin. Has an optimal pH of 7.8-8.5; pI = 9.5. Note: Elastase tends to adhere to glass. Hence, use of siliconized glassware is recommended.
Native elastase from porcine pancreas. A serine protase that catalyzes the hydrolysis of proteins and peptides (especially at bonds adjacent to neutral amino acid residues), including albumin, casein, denatured collagen, elastin, fibrin, and hemoglobin and of a number of synthetic substrates containing aspartic acid, phenylalanine, or tyrosine. Inhibited by DFP, elastinal, and α2-macroglobulin.

包装

1000, 250 u in Plastic ampoule

警告

Toxicity: Standard Handling (A)

单位定义

One unit is defined as the amount of enzyme that will hydrolyze 1.0 mol of Suc-Ala-Ala-Pro-Abu-pNA (Cat no.324699) per min at 25°C, pH 8.0.

外形

Lyophilized from 50 mM Trehalose, 1 mM Acetic Acid.

重悬

Following reconstitution, elastase may be stored at 4°C at pH 6.0 for long term use. If incubated at room temperature at or near its optimum pH, elastase rapidly autolyzes to a mixture of peptides. Stock solutions are stable for up to 2 months at 4°C, pH 6.0.

其他说明

Largman, C. et al. 1976. Biochemestry15, 2491.
Mandl, I. 1962. Methods in Enzymol.5, 665.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

象形图

Exclamation markHealth hazard

警示用语:

Danger

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 3

分析证书

原产地证书 (CofO)

Sanja Blaskovic et al.
American journal of physiology. Lung cellular and molecular physiology, 318(4), L606-L618 (2020-01-23)
Harmful consequences of cigarette smoke (CS) exposure during lung development can already manifest in infancy. In particular, early life exposure to nicotine, the main component of CS, was shown to affect lung development in animal models. We aimed to characterize
Yves Donati et al.
American journal of physiology. Lung cellular and molecular physiology, 318(4), L619-L630 (2020-02-06)
Mouse lung developmental maturation and final alveolarization phase begin at birth. During this dynamic process, alveolar cells modify their morphology and anchorage to the extracellular matrix. In particular, alveolar epithelial cell (AEC) type I undergo cytoplasmic flattening and folding to

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