Merck
All Photos(1)

362280

Sigma-Aldrich

糖蛋白去糖基化试剂盒

manufacturer/tradename

Calbiochem®

质量水平

100

储存条件

do not freeze

储存温度

2-8°C

一般描述

试剂盒包含在中性 pH 下单次反应即可从糖蛋白中去除所有 N-连接,所有简单 O-连接以及几乎所有复杂的 O-连接寡糖所需的所有酶和试剂。适用于天然或变性实验方案,不会降解蛋白质。该试剂盒已组装成包含测序级酶(不含叠氮化物、BSA 或甘油)。每个试剂盒可用于将 2 mg 糖蛋白去糖基化。

包装

1 kit in Fibre case

组分

牛胎球蛋白对照,变性溶液,内-α-N-乙酰半乳糖苷酶,β1,4-半乳糖苷酶,葡萄糖苷酶,N-糖苷酶 F,神经氨酸酶,反应缓冲液,TRITON X-100 洗涤剂和说明书。

警告

毒性:多个毒性值,请参阅 MSDS(O)

产品规格

试验时间:3 h-5 days

其他说明

Chiba, A., et al. 1997.J. Biol. Chem.272, 2156.
Jiang, M.S., and Hart, G.W.1997.J. Biol. Chem.272, 2421.
Wilkins, P.P., et al. 1996.J. Biol. Chem.271, 18732.
Altmann, F., et al. 1995.Glycoconj.J.12, 84.
Szkudlinski, M.W., et al. 1995.
Endocrinology136, 3325.
Pahlsson, P., et al. 1994.Glycoconj.J.11, 43.
Saito, S., et al. 1994.J. Biol. Chem.269, 5644.
Tarentino, A.L., and Plummer, T.H.1994.Methods Enzymol.230, 44.
Iwase, H., Hotta, K. 1993.Methods.Mol.Biol.14, 151.
Stults, N.L., and Cummings, R.D.1993.Glycobiology3, 589.
Hard, K., et al. 1992.Eur. J. Biochem.205, 785.
Kuraya, N., and Hase, S. 1992.J. Biochem.(Tokyo)112, 122.
Trimble, R.B., and Tarentino, A.L.1991.J. Biol. Chem.266, 1646.
Fukuda, M., et al. 1987.J. Biol. Chem.262, 11952.
Sojar, H.T., and Bahl, O.P.1987.Arch.Biochem.Biophys.259, 52.
Taga, E.M., et al. 1984.Biochemistry23, 815.
Kobata, A. 1979.Anal.Biochem.100, 1.
Uchida, Y., et al. 1979.J. Biochem (Tokyo)86, 1573.
Glasgow, L.R., et al. 1977.J. Biol. Chem.252, 8615.
Spiro, R.G., and Bhoyroo, V.D.1974.J. Biol. Chem.249, 5704.
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法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow

警示用语:

Danger

危险分类

Acute Tox. 3 Dermal - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1

储存分类代码

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

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