Merck
All Photos(1)

5.08741

Millipore

DAPI Stain - CAS 28718-90-3 - Calbiochem

Synonym(s):
DAPI Stain - CAS 28718-90-3 - Calbiochem, 4ʹ,6-Diamidino-2-phenylindole, 2HCl, 2-(4-Amidinophenyl)-1H-indole-6-carboxamidine, 2HCl, 2-(4-Carbamimidoylphenyl)-1H-indole-6-carboximidamide, 2HCl, DAPI, ≥97% by HPLC, in H₂O
Empirical Formula (Hill Notation):
C16H15N5 · 2HCl
CAS Number:
Molecular Weight:
350.25
MDL编号:

测定

≥97% (HPLC)

质量水平

200

形式

liquid

manufacturer/tradename

Calbiochem®

储存条件

OK to freeze
avoid repeated freeze/thaw cycles
protect from light

储存温度

−20°C

InChI

1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H

InChI key

FPNZBYLXNYPRLR-UHFFFAOYSA-N

一般描述

Cell-permeable DNA-binding dye. Binds preferentially to DNA rich in adenine and thymine. Used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes. Useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. Can also be used to detect mycoplasma. A reversible inhibitor of S-adenosyl-L-methionine decarboxylase and diamine oxidase. Cells are typically stained at 1 µg/ml.
Cells are typically stained at 1 µg/ml.

生化/生理作用

Cell permeable: yes
Primary Target
DNA
Reversible: yes

包装

Packaged under inert gas

警告

Toxicity: Standard Handling (A)

外形

A 5 mg/ml (14.27 mM) sterile-filtered solution of DAPI, 2HCl (Cat. No. 268298) in H₂O.

重悬

Following initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.

其他说明

Mathieu, C., et al. 1995. Exp. Cell Res.216, 13.
Cubria, C., et al. 1994. Comp. Biochem. Physiol. C.105, 251.
Hotz, M.A., et al. 1994. Cytometry15, 237.
Lee, L.S., and Garnett, H.M. 1993. J. Biochem. Biophys. Methods26, 249.
Miller, T., et al. 1993. Biotechniques15, 1042.

Gray, J.W., et al. 1982. Science238, 323.
Hessling, J.J., et al. 1980. J. Immunol. Methods38, 315.

Russels, W.C., et al. 1975. Nature253, 461.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Skin Sens. 1

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(F)

Not applicable

闪点(C)

Not applicable

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