537088

链霉^®蛋白酶，灰色链霉菌，无核酸酶

Widely used broad-spectrum protease mixture

• CAS Number: 9036-06-0
• MDL编号: MFCD00132092

Properties

• 形式: lyophilized
• specific activity: ≥70,000 proteolytic units/g dry wt
• manufacturer/tradename: Calbiochem^®
• 储存条件: OK to freeze
• 溶解性: water: 10 mg/mL
• 异质活性: Nuclease, none detected
• 运输: ambient
• 储存温度: 2-8°C

Description

一般描述

PRONASE^® Protease, Streptomyces griseus, nuclease-free is a non-specific protease that liquefies mucins and digests proteins to free amino acids. This mixture can be used to isolate living chondrocytes. It is most active at pH 7.0 but stable over wide ranges of pH and temperature. It has an optimal pH of 7.5. Pronase is less cytotoxic and produces more efficient dissociation than collagenase.

应用

Pronase^® Protease, Streptomyces griseus, nuclease-free has been used:

• in single-cell preparations for cardiac side population (CSP) analysis
• in aflatoxin plasma biomarker assay
• to permeabilize Caenorhabditis elegans embryo for in vivo micrococcal nuclease digestion

包装

25 ku in Glass bottle

10 , 100 , 5 , 50 ku in Plastic ampoule

生化/生理作用

Proteases catalyze the hydrolysis of proteins to form peptides and free amino acids. They also regulate the breakdown of fats and carbohydrates through many enzymatic cascades and activating proenzyme zymogens.

警告

毒性：有害（C）

单位定义

一个单位的定义为在40°C、pH 7.5下每分钟释放25 µg酪氨酸当量消化产物所需的酶量。

重悬

溶液中不稳定；仅在使用前进行复溶。

其他说明

注意：1 KU = 1000 单位。

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

PRONASE is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• 象形图: GHS07,GHS08
• 警示用语：: Danger
• 危险声明: H315 - H319 - H334 - H335
• 预防措施声明: P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• 危险分类: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• 靶器官: Respiratory system
• 储存分类代码: 11 - Combustible Solids
• WGK: WGK 2