Merck
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539131

Sigma-Aldrich

蛋白酶抑制剂混合物套装I

A cocktail of five protease inhibitors that will inhibit a broad range of proteases and esterases. Supplied with a data sheet.

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别名:
Protease Inhibitor Mix, Protease inhibitor

形式

lyophilized

制造商/商品名称

Calbiochem®

储存条件

OK to freeze
desiccated (hygroscopic)

运输

wet ice

储存温度

−20°C

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蛋白酶抑制剂混合物套装I A cocktail of five protease inhibitors that will inhibit a broad range of proteases and esterases. Supplied with a data sheet.

539131

蛋白酶抑制剂混合物套装I

蛋白酶抑制剂混合物套装II The Protease Inhibitor Cocktail Set II controls the activity of Protease. This small molecule/inhibitor is primarily used for Protease Inhibitors applications.

539132

蛋白酶抑制剂混合物套装II

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

storage condition

OK to freeze, desiccated (hygroscopic)

storage condition

OK to freeze, desiccated (hygroscopic)

storage condition

OK to freeze, desiccated (hygroscopic)

storage condition

OK to freeze, desiccated (hygroscopic)

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

一般描述

蛋白酶抑制剂混合液Set I A 是一种专门配制的混合液,含有可抑制各种蛋白酶和酯酶的五种不同蛋白酶抑制剂。

特异性

抑制多种蛋白酶和酯酶。

应用

蛋白酶抑制剂混合液 Set I – Calbiochem 已用于裂解缓冲液中以裂解细胞并进行蛋白免疫印迹。
Protease Inhibitor Cocktail Set I have been used as a supplement in:
  • cold radioimmunoprecipitation assay (RIPA) buffer to prepare cell lysates for western blotting
  • RIPA lysis buffer to extract total protein from rat liver tissues and AML12 cells
  • sucrose solution to homogenize mice cortices for myelin isolation

生化/生理作用

Protease inhibitor cocktails and specific protease inhibitors are essential tools utilized by scientists in various proteomic studies. These studies encompass protein expression, characterization, biomarker discovery, mapping post-translational modifications, protein expression profiling, and quantitative protein measurement. The application of a protease inhibitor cocktail or protease inhibitors guarantees the preservation of protein integrity, facilitating downstream analysis and characterization.

特点和优势

  • 蛋白酶抑制剂混合液保护粗提取物中的目标蛋白免于分解
  • 可立即用于重构(reconstitution)

警告

毒性:有害(C)

重悬

复溶后,等分并冷冻保存(-20°C)。贮备溶液在-20°C下可稳定保存至多1个月。
用1 ml dH2O复溶每个小瓶,以获得100X的贮备溶液。

其他说明

如果直接用IMAC树脂处理细胞提取物,EDTA 的存在可能干扰His•Tag 融合蛋白的纯化。

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Eye Irrit. 2

储存分类代码

13 - Non Combustible Solids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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