Merck
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69076

Sigma-Aldrich

pET-32 Ek / LIC矢量试剂盒-Novagen

Novagen′s pET-32 Ek/LIC vector is designed for cloning and high-level expression of target proteins fused with the 109aa Trx-Tag thioredoxin protein and His-Tag and S-Tag thioredoxin protein

质量水平

manufacturer/tradename

Novagen®

储存条件

OK to freeze
avoid repeated freeze/thaw cycles

储存温度

−70°C

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manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

manufacturer/tradename

Novagen®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

storage condition

OK to freeze

storage condition

OK to freeze

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

一般描述

pET-32 Ek/LIC 向量是 pET-32b(+)的 Ek/LIC 版本。它设计用于克隆和高水平表达与 109aa Trx•Tag 硫氧还蛋白融合的目标蛋白,以及可通过肠激酶(Ek)蛋白酶裂解的 His•Tag 和 STag 编码序列。该质粒包含一个强力的 T7lac 启动子,一个优化的 RBS、Ek 蛋白酶切割位点(AspAspAspAspLys↓)的编码序列,以及一个多克隆位点,其中包含在许多其他 Novagen 表达载体中发现的限制酶位点,以促进插入物转移。可选的 C 端 His•Tag 编码序列,便于纯化、检测和定量分析。




本产品的商业使用需要获得 EMD Millipore Corporation 的商业许可。 商业用途应包括但不限于(1)在制造中使用产品或其组件;(2)使用产品或其组件向他人收费提供服务、信息或数据;(3)将产品或其组件(或其任何衍生物)用于治疗、诊断或预防目的(包括作为设备、芯片、检测或其他产品的一部分);(4)转售产品或其组件,无论该产品或其组件是否转售用于研究。 此处包含的任何内容均不应被视为代表或保证使用本产品不需要其他第三方权利。

组分

•1 µgEk/LIC 载体

•10 µlEk/LIC 对照插入片段

•25 UT4 DNA 聚合酶, 经过 LIC 认证

•50 µl10X T4 DNA 聚合酶缓冲液

•100 µl100 mM DTT

•50 µl25 mM EDTA

•40 µl25 mM dATP

•1.5 ml无核酸酶的水

•22 × 50 µlNovaBlue GigaSingles 感受态细胞

•0.2 mlBL21(DE3) 感受态细胞

•0.2 mlBL21(DE3)pLysS 感受态细胞

•5 × 2 mlSOC 培养基

•10 µl测试质粒

警告

毒性:多个毒性值,请参阅 MSDS(O)

法律信息

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

10 - Combustible liquids


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