69079

Sigma-Aldrich

Perfect Protein Markers, 10-225 kDa

storage conditions

-20C

形式

liquid

质量水平

100

manufacturer/tradename

Novagen®

储存条件

OK to freeze
avoid repeated freeze/thaw cycles

运输

wet ice

储存温度

−20°C

相关类别

一般描述

The Perfect Protein<TMSYMBOL></TMSYMBOL> Markers are a novel set of recombinant proteins with definedsizes at convenient intervals. Designed for routine use in SDS-polyacrylamide gelelectrophoresis, the Perfect Protein Markers enable highly accurate size determination ofunknown samples. Unlike many conventional markers (e.g., ovalbumin, serum albumin, etc.), the Perfect Protein Markers contain no oligosaccharides that cause anomalous migration, heterogeneous "fuzzy" bands, or inaccurate size estimation. The markers are optimized for use with Coomassie blue staining, but adjusted amounts can also be used with other gel staining methods (e.g., silver staining, fluorescent dyes, etc.).

The Perfect Protein Markers, 15-150 kDa, include protein sizes of 15, 25, 35, 50, 75,100, and 150 kDa. Each vial contains 400 g protein. A 5-l load contains 0.5 g for each of the protein sizes, except for the 50-kDa band, which contains twice as much protein (1.0 g).

The Perfect Protein Markers, 10-225 kDa, include the protein sizes listed above and two additional proteins, 10 kDa and 225 kDa, for applications requiring a broader size range. Each vial contains 500 g protein. A 5-l load contains 0.5 g for each of the protein sizes, except for the 50-kDa band, which containstwice as much protein (1.0 g).

The 4X SDS Sample Buffer (Cat. No. 70607), which is separately available, is a standard formulation commonly used for SDS-PAGE analysis of proteins. The solution includes DTT for complete denaturation of disulfide bonds. The buffer can be used at a final concentration of 2X for most applications.

包装

100 lane in Glass bottle

特点和优势

• Markers are supplied at the working dilution ingel loading buffer; concentration optimized forCoomassie blue staining

• Recombinant, unmodified markers producesharp, accurately sized bands

• Available in two size ranges:

15-150 kDa

10-225 kDa

警告

Toxicity: Toxic (F)

法律信息

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

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