AB2971

Sigma-Aldrich

抗LAMP-1(CD107a)抗体

from rabbit, purified by affinity chromatography

别名:
lysosomal-associated membrane protein 1, lysosome-associated membrane glycoprotein 1, Lysosome-associated membrane protein 1, CD107 antigen-like family member A, CD107a antigen
eCl@ss:
32160702
NACRES:
NA.41
价格与库存信息目前不能提供

biological source

rabbit

Quality Level

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat, human

species reactivity (predicted by homology)

rhesus macaque (based on 100% sequence homology)

packaging

antibody small pack of 25 μg

application(s)

immunocytochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

General description

溶酶体相关的膜蛋白 1(LAMP-1)是一种高度糖化的单通道 1 型膜蛋白,属于溶酶体相关的膜蛋白家族,该家族是组成溶酶体膜的大部分的一类蛋白,他们与碳水化合物受体相互作用,被认为是细胞粘附的关键介质。 LAMP-1 的功能是将碳水化合物配体转运至选择蛋白。人们认为它还在肿瘤细胞转移中起作用。

Specificity

该抗体识别 LAMP-1 的拓扑结构域(腔)。

Immunogen

表位:拓扑结构域(管腔)
与人 LAMP-1 拓扑结构域(腔)相对应的 KLH 偶联的线性肽。

Application

研究类别
凋亡 & 癌症
蛋白质印迹法(Snap i.d.)分析:1 µg/mL 先前批次的抗体在 10 µg EL4 细胞裂解液中检测到 LAMP-1。

免疫细胞化学分析: 先前批次的抗体以 1:500 的稀释度在 NIH/3T3、A431 和 HeLa 细胞中检测到 LAMP-1。
研究子类别
细胞凋亡 - 其他

肿瘤标记物
该抗 LAMP-1(CD107a)抗体经过验证可用在 WB 和 IC 中检测 LAMP-1(CD107a)。

Quality

通过蛋白质印迹在 EL4 细胞裂解液中进行了评估。

蛋白质印迹分析:1 µg/mL 的该抗体在 10 µg EL4 细胞裂解液中检测到 LAMP-1。

Target description

观测值〜95kDa。 尽管计算得出的分子量为 45 kDa,但 LAMP-1 被高度糖基化,因此,预期典型的条带在 90-120 kDa 之间。在某些裂解液中可能会观察到未表征的条带。

Physical form

亲和纯化
在含 0.1 M Tris-甘氨酸(pH 7.4),150 mM NaCl 和 0.05% 叠氮化钠的缓冲液中的纯化的兔多克隆抗体。

Storage and Stability

自收到之日起,在 2-8°C 条下可稳定保存 1 年。

Analysis Note

对照
EL4 细胞裂解液

Other Notes

浓度:请参考批次特异性浓缩物的分析证书。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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