Interferon regulatory factor 1 (UniProt: P10914; also known as IRF-1) is encoded by the IRF1 gene (Gene ID: 3659) in human. IRF-1 is a transcriptional regulator that is expressed constitutively in all cells types except early embryonal cells. It can exist as a monomer and as a homodimer. It displays remarkable functional diversity in the regulation of cellular responses, including the regulation of interferon (IFN) and IFN-inducible genes, host response to viral and bacterial infections. It also regulates many genes expressed during hematopoiesis, inflammation, immune responses, cell proliferation and differentiation, and growth arrest and apoptotic cell death following DNA damage. IRF-1 is reported to stimulate p53/TP53-dependent transcription through enhanced recruitment of EP300 leading to increased acetylation of p53/TP53. Its DNA-binging domains are located in the first 115 amino acids of N-terminal region and contains a series of five well-conserved tryptophan-rich repeats. IRF-1 can be phosphorylated by casein kinase 2, which positively regulates its activity. Mutations in IRF1 gene are reported to cause gastric cancer. (Ref.: Dou, L., et al. (2014). Hum. Immunol. 75(11); 1110-1114).
This rabbit polyclonal antibody detects Interferon Regulaotry Factor 1 in human cells. It targets an epitope within 14 amino acids from the N-terminal half.
KLH-conjugated linear peptide corresponding to 14 amino acids from the N-terminal half of human Interferon Regulatory Factor 1.
Western Blotting Analysis: A 1:500 dilution from a representative lot detected IRF-1 in Jurkat cell lysate.
Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected IRF-1 in human stomach cancer and human small intestine tissue sections.
Anti-IRF-1, Cat. No. ABF188, is a rabbit polyclonal lantibody that detects Interferon regulatory factor 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Inflammation & Immunology
Evaluated by Western Blotting in PC12 cell lysate.
Western Blotting Analysis: A 1:500 dilution of this antibody detected IRF-1 in PC12 cell lysate.
~48 kDa observed; 36.50 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
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