ABN1388

Sigma-Aldrich

Anti-MUL1

from rabbit, purified by affinity chromatography

别名:
Mitochondrial ubiquitin ligase activator of NFKB 1, E3 SUMO-protein ligase MUL1, E3 ubiquitin-protein ligase MUL1, Growth inhibition and death E3 ligase, Mitochondrial-anchored protein ligase, MAPL, Putative NF-kappa-B-activating protein 266, RING finger
eCl@ss:
32160702
NACRES:
NA.41
价格与库存信息目前不能提供

biological source

rabbit

Quality Level

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

rhesus macaque (based on 100% sequence homology)

packaging

antibody small pack of 25 μg

application(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

ambient

General description

Mitochondrial ubiquitin ligase activator of NFKB 1 (UniProt: Q969V5; also known as E3 SUMO-protein ligase MUL1, E3 ubiquitin-protein ligase MUL1, Growth inhibition and death E3 ligase, Mitochondrial-anchored protein ligase, MAPL, MAPL, Putative NF-kappa-B-activating protein 266, RING finger protein 218) is encoded by the MUL1 (also known as C1orf166, GIDE, MAPL, MULAN, RNF218) gene (GeneID: 79594) in human. MUL1 is a multi-pass membrane protein located on the outer mitochondrial membrane. It plays a role in the control of mitochondrial morphology and also promotes mitochondrial fragmentation and influences mitochondrial localization. MUL1 displays a weak E3 ubiquitin-protein ligase activity. It contains a RING-type zinc finger domain (aa 302-340), which is required for its E3 ligase activity. MUL1 can ubiquitinate Akt1 preferentially at ′Lys-284′ involving ′Lys-48′-linked polyubiquitination and seems to be involved in regulation of Akt signaling by targeting phosphorylated Akt to proteosomal degradation. When over-expressed, MUL1 is reported to inhibit cell growth by activating JNK through MAP3K7/TAK1 and inducing caspase-dependent apoptosis. MUL1 is ubiquitinated by PARK2 during mitophagy, leading to its degradation and enhancement of mitophagy.

Specificity

This rabbit polyclonal antibody detects Mitochondrial ubiquitin ligase activator of NFKB 1 in Human skeletal muscle tissue.

Immunogen

KLH-conjugated linenar peptide corresponding to 12 amino acids from the C-terminal region of human Mitochondrial ubiquitin ligase activator of NFKB 1 (MUL1).

Application

Research Category
Neuroscience
Immunohistochemistry Analysis: A 1:50-250 dilution of this antibody detected MUL1 in human cerebellum, human skeletal muscle myocytes, and human cerebral cortex.
Anti-MUL1, Cat. No. ABN1388, is a highly specific rabbit polyclonal antibody that targets Mitochondrial ubiquitin ligase activator of NFKB 1 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Quality

Evaluated by Western Blotting in human skeletal muscle tissue lysate.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected MUL1 in 10 µg of human skeletal muscle tissue lysate.


Target description

~39 kDa observed; 39.8 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity Purified

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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