ABN1739M

Sigma-Aldrich

Anti-Utrophin A

from rabbit

别名:
UTRN A
eCl@ss:
32160702
NACRES:
NA.41
价格与库存信息目前不能提供

biological source

rabbit

Quality Level

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, human

packaging

antibody small pack of 25 μg

application(s)

immunofluorescence: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG

NCBI accession no.

UniProt accession no.

Gene Information

human ... UTRN(7402)

General description

Utrophin A (UniProt: E9Q6R7) is encoded by the Utrn gene in murine species. Utrophin is a large cytoskeletal protein, which is autosomally-encoded homologue of dystrophin, the protein product of the Duchenne muscular dystrophy (DMD) gene. It displays a sequence homology with dystrophin and possesses many of the protein-binding properties ascribed to dystrophin. In normal skeletal muscle, utrophin is located at the neuromuscular junction and dystrophin predominates at the sarcolemma. During development and in certain myopathies utrophin is also reported to be present at the sarcolemma and this redistribution is associated with higher levels of utrophin. Utrophin is found to co-localize with the acetylcholine receptors at the neuromuscular junctions and may participate in stabilizing the synaptic cytoskeleton. The utrophin mRNA contains two full-length species (named A- and B-utrophin), which are transcribed from different promoters. Hence, utrophin is a composite of A- and B-utrophin and only the A-utrophin is up-regulated in dystrophin-deficient striated muscle. This up-regulation is reported to occur post-transcriptionally. Utrophin A and B differ in their N-termini sequence. (Ref.: Blake, DJ et al. (1996). Brain Pathol. 6(1); 37-47; Weir, AP et al. (2002). J. Biol. Chem. 277 (47); 45285-90).

Specificity

This rabbit polyclonal antibody detects utrophin in human and mouse muscle tissue. It targets an epitope with in the first 26 amino acids from the N-terminal region.

Immunogen

Epitope: N-terminus
KLH-conjugated linear peptide corresponding to the first 26 amino acids from the N-terminal region of murine utrophin.

Application

Anti-Utrophin A Antibody, Cat. No. ABN1739, is a highly specific rabbit polyclonal antibody that targets Utrophin and has been tested in Immunofluorescence, Immunohistochemistry (Paraffin), and Western Blotting.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Utrophin A in human skeletal muscle and human uterus tissue.

Immunofluorescence Analysis: A representative lot detected Utrophin A in quadriceps transverse sections in transgenic mice (Adams, M.E., et. al. (2010). J Neurosci. 30(33):11004-10).

Immunohistochemistry Analysis: A representative lot detected Utrophin A in frozen sections of the gastrocnemius muscles (Banks, G.B., et. al. (2014). PLoS Genet. 10(6):e1004431).

Western Blotting Analysis: representative lot detected Utrophin A in whole muscle lysates (Banks, G.B., et. al. (2014). PLoS Genet. 10(6):e1004431).

Western Blotting Analysis: A representative lot detected Utrophin A in muscle membrane extracts from mdx mice (Courtesy of Dr Marvin Adams at University of Washington).

Immunofluorescence Analysis: A representative lot detected Utrophin A in mouse neuromuscular junction (Courtesy of Dr Marvin Adams at University of Washington).
Research Category
Neuroscience

Quality

Evaluated by Immunohistochemistry in human prostate.

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Utrophin A in human prostate tissue.

Target description

394.47 kDa calculated.

Physical form

Format: Purified
Affinity Purified
Purified rabbit polyclonal antibody in PBS with 0.02% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Flash Point(F)

does not flash

Flash Point(C)

does not flash

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