Vang-like protein 2 (UniProt: Q9ULK5; also known as Loop-tail protein 1 homolog, Strabismus 1, Van Gogh-like protein 2) is encoded by the VANGL2 (also known as KIAA1215, STB1) gene (Gene ID: 57216) in human. Vangl2 is a homodimeric multi-pass membrane protein that plays a role in the control of early morphogenesis, patterning of both axial midline structures and the development of neural plate. It can also form heterodimer with VANGL1. It is required for polarization and movement of myocardializing cells in the outflow tract and seems to act via RHOA signaling to regulate this process. Vang-like protein 2 is reported to bind to Dvl to cell-autonomously promote efficient Dvl plasma membrane recruitment, a pre-requisite for planar cell polarity activation. At the same time, it can inhibit Dvl from interacting with its downstream effector (Dishevelled associated activator of morphogenesis 1 (Daam1), and functionally suppresses Dvl - Daam1 cascade. Mutations in VANGL2 gene cause neural tube defects that results in defective neural tube closure during the first trimester of pregnancy. (Ref.: Seo, HS et al. (2017). Hum. Mol. Genet. 26(11); 2053-2061).
This rabbit polyclonal antibody detects Vang-like protein 2 in human and murine cells. It targets an epitope with in 15 amino acids from the N-terminal region.
KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal region of human Vang-like Protein 2.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Vangl2 in HT1080 cells.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Vangl2 in human tonsil and mouse embryonic spinal cord tissue sections.
Anti-Vangl2, Cat. No. ABN2242, is a highly specific rabbit polyclonal antibody that targets Vang-like protein 2 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Evaluated by Western Blotting in HepG2 cell lysate.
Western Blotting Analysis: 2 µg/mL of this antibody detected Vangl2 in HepG2 cell lysate.
~60 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
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