ABT45

Sigma-Aldrich

抗-Claudin-5抗体

from rabbit, purified by affinity chromatography

别名:
Claudin-5, Transmembrane protein deleted in VCFS, TMDVCF
eCl@ss:
32160702
NACRES:
NA.41
价格与库存信息目前不能提供

biological source

rabbit

Quality Level

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

mouse, rat, horse, human

species reactivity (predicted by homology)

equine (based on 100% sequence homology), ox (based on 100% sequence homology)

packaging

antibody small pack of 25 μg

application(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

General description

Claudin-5是一种在心肺面综合征(TMVCF)中缺失的跨膜蛋白,可能与内皮紧密连接有关。Claudin-5仅在内皮细胞的细胞边界中发现。肝损伤期间窦状内皮细胞中较低的claudin-5水平可能导致其功能障碍。肿瘤血管中claudin-5的水平可能是肝细胞癌预后的潜在标志物。Claudin-5功能的丧失与致癌作用有关。

Specificity

该抗体可识别Claudin-5的胞质结构域。

Immunogen

与人Claudin-5细胞质结构域对应的KLH缀合线性肽。
表位:细胞质结构域

Application

研究子类别
粘附力(CAMs)
研究类别
细胞结构
免疫组化分析:一个代表性批次产品以1:800的稀释度在前列腺组织中检测到Clausin-5。
抗Claudin-5抗体可检测Claudin-5水平 & 已出版&并经过验证可用于WB、IH(P)。

Quality

已通过蛋白质印迹法在人肺组织裂解液中进行了评估。

蛋白质印迹分析:0.1 µg/mL的该抗体在10 µg人肺组织裂解液中检测到Claudin-5。

Target description

观测值〜17 kDa。
由于磷酸化作用和糖基化作用,可以观察到〜17 kDa和62 kDa的分子量(Ishizaki, T., et al. (2003).Experimental Cell Research.290:275–288).

Physical form

亲和纯化
在含 0.1 M Tris-甘氨酸(pH 7.4),150 mM NaCl 和 0.05% 叠氮化钠的缓冲液中的纯化的兔多克隆抗体。

Storage and Stability

自发运之日起,在 2-8°C 条件下可稳定保存1年

Analysis Note

对照
人肺组织裂解液

Other Notes

浓度:请参考批次特异性浓缩物的分析证书。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Hongyan Zhu et al.
Neurochemical research, 43(10), 1897-1904 (2018-08-09)
Blood-brain barrier (BBB) destruction is associated with a variety of neurological diseases. Brain microvascular endothelial cells (BMECs) are the key constituent of BBB. Both matrix metalloproteinases-2/9 (MMP-2/9) and toll-like receptor-2 (TLR2) are coexpressed in BMECs and have been shown to...
Disrupted tight junctions in the small intestine of cystic fibrosis mice.
De Lisle, RC
Cell and Tissue Research null
Karsten Bartels et al.
Heart, lung & circulation, 23(10), 981-984 (2014-06-17)
Neurologic injury is common after cardiac surgery and disruption of the blood brain barrier (BBB) has been proposed as a contributing factor. We sought to study BBB characteristics in a rodent model of cardiopulmonary bypass (CPB) and deep hypothermic circulatory...
Narin Liman et al.
Animal reproduction science, 212, 106247-106247 (2019-12-23)
Claudins (CLDNs) are major Ca2+-independent cell adhesion molecules functioning at tight junctions (TJ). The presence and localization of cell adhesion molecules are important for understanding the mechanisms associated with follicular and luteal development in the ovary. In this study, there...
Neutrophil elastase contributes to the pathological vascular permeability characteristic of diabetic retinopathy.
Liu, et al.
Diabetologia, 62, 2365-2374 (2020)

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