Merck
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AP1064

Sigma-Aldrich

PhosphoDetect 抗-PDH-E1α (pSer³⁰⁰)兔多克隆抗体

liquid, Calbiochem®

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别名:
抗丙酮酸脱氢酶pSer³⁰⁰兔pAb

生物来源

rabbit

质量水平

抗体形式

affinity isolated antibody

antibody product type

primary antibodies

克隆

polyclonal

形式

liquid

包含

≤0.1% sodium azide as preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

储存条件

OK to freeze
avoid repeated freeze/thaw cycles

运输

wet ice

储存温度

−20°C

target post-translational modification

phosphorylation (pSer300)

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此商品
PC386PS1019527460
antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

-

antibody form

-

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

form

liquid

form

liquid

form

liquid

form

liquid

species reactivity

human

species reactivity

rat, mouse, human

species reactivity

mouse, rat, human

species reactivity

human, mouse

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

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一般描述

免疫亲和纯化的兔多克隆抗体。可识别出在Ser300处磷酸化的~44 kDa的PDH-E1α蛋白。
识别HEK293细胞中在Ser300磷酸化的~44 kDa PDH-E1α蛋白。
该PhosphoDetect抗-PDH-E1α pSer³⁰⁰)兔pAb经验证可用于免疫印迹法、免疫细胞化学检测PDH-E1α(pSer³⁰⁰)。

免疫原

合成磷酸肽,对应于人PDH-E1的Ser³⁰⁰磷酸化位点周围的氨基酸α

应用



免疫印迹(0.5μg/ml)
免疫细胞化学(1μg/ml)

包装

请参考特定浓度批号的标签。

警告

毒性:标准处理(A)

外形

在150 mM NaCl,100 mM Tris-甘氨酸,pH 7.4中。

重悬

初次融化后,等分并冷冻(-20°C)。

分析说明

对照
HEK293细胞
阴性对照
用二氯乙酸盐处理的HEK293细胞

其他说明

Rardin M.J., et. al. 2009.Anal.Biochem.2, 157.
Seifert, F., et al. 2007.Biochemistry 21, 6277.
Lee, J., et al. 2007.Mol.Cell Prot.4, 669.
Patel, M.S. and Korotchkina, L.G.2006 Biochem.Soc.Trans.34, 217.
Korotchkina, L.G., et al. 2001.J. Biol. Chem. 40, 37223.
由于高程度序列一致性,该抗体也预期与小鼠,大鼠,牛,斑马鱼和非洲爪蟾发生交叉反应。磷酸化PDH-E1α抗体应进行滴定以在单个系统中获得最佳结果。

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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