Merck
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CBL404

Sigma-Aldrich

Anti-p53 Antibody, aa 211-220, clone240

clone PAb240, Chemicon®, from mouse

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eCl@ss:
32160702
NACRES:
NA.41

生物来源

mouse

质量水平

抗体形式

purified antibody

antibody product type

primary antibodies

克隆

PAb240, monoclonal

species reactivity

hamster, monkey, mouse, chicken, human, rat, bovine

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable

同位素/亚型

IgG1

适用性

not suitable for immunohistochemistry (Paraffin)

NCBI登记号

UniProt登记号

运输

wet ice

Gene Information

human ... TP53(7157)

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此商品
MABE339SAB1406544SAB4503015
antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

clone

PAb240, monoclonal

clone

Pab1620, monoclonal

clone

polyclonal

clone

polyclonal

species reactivity

hamster, monkey, mouse, chicken, human, rat, bovine

species reactivity

human, mouse

species reactivity

human

species reactivity

mouse, human, rat

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

technique(s)

immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunohistochemistry: suitable, western blot: suitable

technique(s)

indirect immunofluorescence: suitable, western blot: 1 μg/mL

technique(s)

ELISA: 1:10000, immunofluorescence: 1:100-1:500, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

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一般描述

p53 was discovered in 1979 as a cellular protein associating with the transforming protein of SV40 tumor virus. Since then, many different biochemical functions have been attributed to the 53 kD phosphoprotein. Experimental evidence has suggested that p53 acts as a negative regulator of cell growth in normal cells (Finlay, 1989). Thus, the inactivation or mutation of p53 may be an essential step in the development of malignancy (Lane and Benchmol, 1990). Wild-type p53 levels in normal cells and tissues were found to be very low. Mutant p53 polypeptide, however, is often found to be present at high concentrations in mammalian tumors and tumor cell lines. For example, in an immuno-histochemistry study 40% of human breast cancer showed elevated levels of mutant p53 in the cell nucleus. Mutations of the p53 protein have some characteristic features:

a) Most of them are missense point mutations giving rise to an altered protein function.

b) Many -but not all- mutant p53 proteins exhibit a common mutant structure, which can be recognized by monoclonal antibodies specific for p53 in the mutant conformation.

特异性

PAb 240 recognizes an epitope of p53 tumor suppressor protein between amino acids 211 and 220 (Gannon, 1990; Legros, 1994) in human, mouse, rat, hamster, monkey, cow and chicken. Assaying native samples (immunoprecipitation, ELISA) the PAb 240 detects only the mutant forms of p53 (Gannon, 1990; Said, 1994). In methods using denatured samples [Western blot analysis (Gannon, 1990) and immunohistochemistry of frozen tissue sections (Said, 1994; Bartek, 1991; Walker, 1991)], the PAb 240 recognizes both mutant and denatured wild-type p53.

免疫原

Epitope: aa 211-220
Murine p53-beta galactosidase fusion protein expressed in E. coli.

应用

Detect p53 with Anti-p53 Antibody, aa 211-220, clone240 (Mouse Monoclonal Antibody), that has been shown to work in IP, WB & IHC.
Detection of p53 oncogene protein

Detection of mutant p53

Prevalence of detection using CBL 404

-50% colon carcinoma sections positive (30 samples)

-70% lung carcinoma sections positive (50 samples)

-30% carcinoma breast samples positive (50 samples)

Normal and pre-malignant tissues negative

Reacts on methacarn fixed tissue

Optimal working dilutions must be determined by the end user.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Transcription Factors

联系

Replaces: 04-1083

外形

Format: Purified
Purified mouse monoclonal in buffer containing 0.1M Tris-glycine (pH 7.4), 0.15M NaCl, with 0.05% sodium azide. We recommend that each laboratory determine an optimum working titre for use in its particular application.

储存及稳定性

For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Siddharth Singh et al.
The Journal of biological chemistry, 294(38), 14081-14095 (2019-08-02)
TP53 is the most frequently mutated tumor suppressor gene in many cancers, yet biochemical characterization of several of its reported mutations with probable biological significance have not been accomplished enough. Specifically, missense mutations in TP53 can contribute to tumorigenesis through
Rajan Gogna et al.
The Journal of biological chemistry, 287(4), 2907-2914 (2011-12-08)
Mutant (Mt) p53 abrogates tumor suppression functions of wild-type (WT) p53 through mutant-specific, gain-of-function effects, and patients bearing Mt p53 are chemoresistant. The dominant negative effect of p53 mutants results from their aggregation propensity which causes co-aggregation of WT p53.
Yongxing Wang et al.
The Journal of clinical investigation, 121(3), 893-904 (2011-02-03)
The transcription factor p53 is a tumor suppressor. As such, the P53 gene is frequently altered in human cancers. However, over 80% of the P53 mutations found in human cancers are missense mutations that lead to expression of mutant proteins
Increased expression of mutant forms of p53 oncogene in primary lung cancer.
Iggo, R, et al.
Lancet, 335, 675-679 (1990)
Linear antigenic sites defined by the B-cell response to human p53 are localized predominantly in the amino and carboxy-termini of the protein.
Legros, Y, et al.
Oncogene, 9, 2071-2076 (1994)

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