Merck
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DP02

Sigma-Aldrich

抗SV40 T抗原(Ab-2)小鼠单克隆抗体(PAb416)

liquid, clone PAb416, Calbiochem®

生物来源

mouse

质量水平

抗体形式

purified antibody

antibody product type

primary antibodies

克隆

PAb416, monoclonal

形式

liquid

包含

≤0.1% sodium azide as preservative

species reactivity

SV40-infected cells

manufacturer/tradename

Calbiochem®

储存条件

do not freeze

同位素/亚型

IgG2a

运输

wet ice

储存温度

2-8°C

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This Item
DP02AOP33MABF121
biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

antibody form

purified antibody

clone

PAb416, monoclonal

clone

PAb416, monoclonal

clone

PAb1620, monoclonal

clone

PAb416, monoclonal

form

liquid

form

slurry (Liquid)

form

liquid

form

-

species reactivity

SV40-infected cells

species reactivity

SV40-infected cells

species reactivity

human, mouse, rat, primate, bovine

species reactivity

SV40-infected cells

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

一般描述

SV40 T Ag(Ab-2)是一种小鼠单克隆抗体,对SV40大T抗原特有的抗原决定簇具有特异性,与SV40小T抗原无反应性。两种抗原均由SV40基因组的早期区域编码。大的T抗原结合DNA,并与53,000道尔顿的细胞转化蛋白p53形成复合物,p53是裂解生长过程中启动病毒DNA复制所必需的。此外,大T抗原与DNA聚合酶和转录因子AP-2结合,并与视网膜母细胞瘤易感基因的p105产物形成特异性复合物。
识别SV80和SV-T2细胞中的~94 kDa SV40大T抗原。
该抗SV40 T抗原(Ab-2)小鼠mAb(PAb416)经验证可用于亲和纯化、免疫印迹、IF、IP和石蜡切片,以检测SV40 T抗原(Ab-2)。
通过用指定的免疫原免疫小鼠并将脾细胞与NS-1小鼠骨髓瘤融合而产生的纯化的小鼠单克隆抗体(请参阅应用参考)。识别~94 kDa SV40大T抗原。

免疫原

纯化SV40大T抗原
表位:氨基酸83-128内

应用

亲和纯化(使用目录编号DP02A)

免疫印迹(1-5 µg/ml,请参阅应用参考)

免疫荧光(1-5 µg/ml)

免疫沉淀(1-5 µg抗体,使用目录编号DP02A)

石蜡切片(请参阅应用参考)

包装

请参考特定浓度批号的标签。

警告

毒性:标准处理(A)

外形

溶于50 mM磷酸钠缓冲液(pH 7.5),含0.2%明胶。

分析说明

阳性对照
SV80或SV-T2细胞
阴性对照
3T3细胞

其他说明

DeCaprio, J.A., et al. 1988.Cell54, 275.
Whyte, P., et al. 1988.Nature334, 124.
Mitchell, P.J., et al. 1987.Cell50, 847.
Dixon, R.A.F. and Nathans, D., 1985.J. Virol.53, 1001.
Simanis, V. and Lane, D.P.1985.Virol.144, 88.
Mann, R.S. and Carroll, R.B.1984.Virology138, 379.
Sarnow, P., et al. 1982.Cell28, 387.
Crawford, L.V., et al. 1981.Proc.Natl.Acad.Sci. USA78, 41.
Lane, D.P. and Crawford, L.V.1979.Nature278, 261.
Carroll, R.B., et al. 1974.Proc.Natl.Acad.Sci. USA71, 3754.
Tooze, J. 1973.Cold Spring Harbor, New York.
Tegtmeyer, P. 1972.J. Virol.10, 591.
也可用琼脂糖偶联物(目录编号DP02A),适用于亲和纯化和免疫沉淀。在单个系统中,应对抗体进行滴定以获得最佳结果。

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(F)

Not applicable

闪点(C)

Not applicable


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