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HTS020M

Sigma-Aldrich

ChemiSCREEN Membrane Preparation Recombinant Human CB2 Cannabinoid Receptor

Human CB2 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.

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别名:
Cannabinoid receptor membrane prep, Human CB2 membrane prep
eCl@ss:
32161000
NACRES:
NA.41

生物来源

human

质量水平

重组

expressed in CHO cells

制造商/商品名称

ChemiScreen
Chemicon®

技术

ligand binding assay: suitable (GTPγS)
radioligand binding assay (RLBA): suitable

NCBI登记号

UniProt登记号

运输

dry ice

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此商品
HTS019MHTS182MHTS138M
ChemiSCREEN CB1 Membrane Preparation Human CB1 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.

HTS019M

ChemiSCREEN CB1 Membrane Preparation

recombinant

expressed in CHO cells

recombinant

expressed in Chem-1 cells

recombinant

expressed in Chem-1 cells

recombinant

expressed in Chem-1 cells

manufacturer/tradename

ChemiScreen

manufacturer/tradename

ChemiScreen, Chemicon®

manufacturer/tradename

ChemiScreen, Chemicon®

manufacturer/tradename

ChemiScreen, Chemicon®

technique(s)

ligand binding assay: suitable (GTPγS)

technique(s)

ligand binding assay: suitable (GTPγS), radioligand binding assay (RLBA): suitable

technique(s)

ligand binding assay: suitable (GTPγS), radioligand binding assay (RLBA): suitable

technique(s)

ligand binding assay: suitable (GTPγS), radioligand binding assay (RLBA): suitable

NCBI accession no.

NM_001841.1

NCBI accession no.

NM_016083.3, NM_033181.2

NCBI accession no.

NM_144773.2

NCBI accession no.

NM_020311.2

UniProt accession no.

P34972

UniProt accession no.

P21554

UniProt accession no.

Q8NFJ6

UniProt accession no.

P25106

一般描述

Cannabinoid compounds include exogenous drugs such as D9-THC, the main psychoactive component of the plant Cannabis sativa, and endogenous mediators, such as anandamide, that belong to eicosanoid family.The biological effects of cannabinoids are mediated by a family of two Gi-coupled 7-transmembrane receptors, CB1 and CB2. The CB1 receptor is found primarily in brain and mediates the psychoactive effects of cannabinoid ligands. The CB2 receptor is expressed mainly in immune cells, including mast cells and CD40-activated B cells, where it mediates proliferation and inhibition of migration (Howlett et al., 2002). Activation of CB2 inhibits the development of liver fibrosis (Julien et al., 2005). In bone, CB2 is expressed in both osteoblasts and osteoclasts, and functions to prevent bone loss (Ofek et al., 2006). In addition, activation of CB2 has an antinociceptive effect in animal models of neuropathic, inflammatory, and acute pain; this effect is mediated by release of endogenous opioids in the periphery (Ibrahim et al., 2005). Millipore′s CB2 membrane preparations are crude membrane preparations made from stable recombinant cell lines with a high-level of GPCR surface expression; thus, they are ideal HTS tools for screening for agonists and antagonists of CB2. The membrane preparations exhibit a Kd of 2.7 nM for [3H]-CP55,940. With 5 μg/well CB2 Membrane Prep and 2.5 nM [3H]-CP55,940, greater than 10-fold signal-to-background ratio is obtained.
Full-length Human CB2

质量

Table 1. Signal:background and specific binding values obtained in a competition binding assay with varying amounts of CB2 membrane prep.
10 µg/well 5 µg/well
Signal:Background 33.5 25.2
Specific Binding (cpm) 7336 6207

SPECIFICATIONS: 1 unit = 5 μg membrane preparation
Bmax: 50 pmol/mg
Kd: 2.7 nM

产品规格

Inucbation Conditions
RECOMMENDED ASSAY CONDITIONS: Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.

Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C.

Radioligand: [3H]-CP55940 (Perkin Elmer #NET1051)

Wash Buffer: 50 mM Hepes, pH 7.4, 500 mM NaCl , 0.1% BSA, filtered and stored at 4°C.

One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 10-fold signal:background with 3H-labeled CP55,940 at 2.5 nM

外形

Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA no preservatives.
Packaging method: Membranes proteins were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Alexei Yeliseev et al.
Scientific reports, 11(1), 3706-3706 (2021-02-14)
Signaling through integral membrane G protein-coupled receptors (GPCRs) is influenced by lipid composition of cell membranes. By using novel high affinity ligands of human cannabinoid receptor CB2, we demonstrate that cholesterol increases basal activation levels of the receptor and alters

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