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HTS095RTA

Sigma-Aldrich

Ready-to-Assay Kappa Opioid Receptor Frozen Cells

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eCl@ss:
32011203

生物来源

human

质量水平

制造商/商品名称

Ready-to-Assay

技术

calcium flux assay: suitable

NCBI登记号

检测方法

fluorometric

运输

dry ice

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此商品
HTS101RTAHTS100RTAHTS233LRTA
technique(s)

calcium flux assay: suitable

technique(s)

calcium flux assay: suitable

technique(s)

calcium flux assay: suitable

technique(s)

activity assay: suitable (cAMP), calcium flux assay: suitable

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

manufacturer/tradename

Ready-to-Assay

manufacturer/tradename

Ready-to-Assay

manufacturer/tradename

Ready-to-Assay

manufacturer/tradename

Ready-to-Assay

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

NCBI accession no.

NM_000912

NCBI accession no.

NM_000914

NCBI accession no.

NM_000911

NCBI accession no.

-

一般描述

EMD Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. EMD Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

Opiates derived from the opium poppy, Papaver somniferum, have been used for millenia for their anti-diarrheal, analgesic and euphoric properties. More recently, endogenous peptides, enkephalins, dynorphins, and endorphins, were found to bind to the same sites as opiate alkaloids. The receptors for the classical opioids are three related GPCRs, μ, κ, and d, that activate Gi/o to reduce intracellular cAMP levels. Most clinically used opioids function by activation of the μ opioid receptor. Activation of the κ opioid receptor by selective agonists also produces analgesia, primarily mediated by spinal sites, but causes dysphoria and psychosis instead of euphoria. The κ receptor at central and peripheral sites is also largely responsible for the anti-diarrheal effects of opiates (Dhawan et al., 1996). EMD Millipore’s cloned human κ-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant κ expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway.

细胞系描述

GPCR Cell Lines
Host cells: Chem-1

生化/生理作用

GPCR Class: A
Protein Target: Kappa / OP2 / KOP / KOR
Target Sub-Family: Opioid

组分

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.

Fifty (50) mL of Media Component.

其他说明

Limited to a single use and shall not be propagated and/or re-frozen by licensee.

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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