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MAB1217F

Sigma-Aldrich

Anti-FMC-7 B-Cell Lymphocyte Marker Antibody, clone FMC-7, FITC conjugated

clone FMC-7, Chemicon®, from mouse

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eCl@ss:
32160702
NACRES:
NA.41

生物来源

mouse

质量水平

偶联物

FITC conjugate

抗体形式

purified immunoglobulin

克隆

FMC-7, monoclonal

种属反应性

human

制造商/商品名称

Chemicon®

技术

flow cytometry: suitable

同位素/亚型

IgM

运输

wet ice

靶向翻译后修饰

unmodified

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FCMAB174FFCMAB192FFCMAB184F
species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

conjugate

FITC conjugate

clone

FMC-7, monoclonal

clone

CBC.37, monoclonal

clone

Ber-H2, monoclonal

clone

HD37, monoclonal

antibody form

purified immunoglobulin

antibody form

purified antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

shipped in

wet ice

一般描述

B cells are lymphocytes that play a large role in the humoral immune response as opposed to the cell-mediated immune response that is governed by T cells. The principal function of B cells is to make antibodies against soluble antigens. B cells are an essential component of the adaptive immune system. FMC-7 B-Cell Lymphocyte Marker is a 105 kDa B cell restricted antigen which is expressed on about 50% of adult human peripheral blood B cells. Upon in vivo B cell activation FMC7 expression initially increases. B cells involved in antibody secretion have lost the FMC7 determinant. The monoclonal antibody FMC7 delineates a subpopulation of B lymphocytes in normal blood. Expression of the antigen recognized by FMC7 appears to be maturation-linked, and it serves to distinguish different types of B cell leukemia.
BIOCHEMISTRY: p I = 7.4

特异性

The FMC-7 antibody was not clustered at the 5th Leucocyte Typing Workshop held in Boston USA 1993. This antibody detects a glycoprotein of 105kD found on circulating B-lymphocytes (Brooks et al., 1981). Studies on normal lymphocytes, leukaemic cells and cell lines indicate that FMC-7 is a marker for a limited segment of the B cell maturation pathway (Catovsky et al., 1981; Zola et al., 1984). This glycoprotein is expressed in varying degrees on normal circulating B-cells, chronic B-cell leukaemia (B-CLL), prolymphocytic leukaemia (PLL) and other B cell neoplasias (Zola et al., 1984, 1987; Bloem et al., 1988; Melo et al., 1988; Ferro & Zola, 1990; Mulligan, 1990; Zola et al., 1991; Burthem & Cawley, 1994; Huh et al., 1994). It labels the same population as CD22 antibodies but reacts with a different antigen (Zola et al. 1987).

Cell reactivity:1

Stains peripheral blood B lymphocytes and tonsil B lymphocytes. No reaction with granulocytes, monocytes, platelets, erythrocytes, T lymphocytes or null cells. Reacts with HRIK and Raji cell lines.

Clinical9,12,13 Expression

B cell prolymphocytic leukemia (B-PLL) Strongly positive

Hairy cell leukemia (HCL) Strongly positive

Hairy cell leukaemia variant (HCL-V) Strongly positive

Splenic lymphoma with villous lymphocytes (SLVL) Positive

B cell chronic lymphocytic leukaemia (B-CLL) Negative to weakly positive

免疫原

The human B-lymphoblastoid cell line, HRIK.

应用

Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This Anti-FMC-7 B-Cell Lymphocyte Marker Antibody, clone FMC-7, FITC conjugated is validated for use in Flow Cytometry (FC) for the detection of FMC-7 B-Cell Lymphocyte Marker.
This antibody can be used for the differentiation of PLL from B-CLL. It is also useful in confirmation of the diagnosis of other disorders such as HCL, HCL-V and SLVL. Useful for the detection of residual disease in patients undergoing treatment for these disorders.


SUGGESTED USAGE:

Flow cytometry - use 10 μl direct from the vial per 100 μl of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.

目标描述

105 kDa

联系

Replaces: CBL1416F

外形

The antibody is supplied in 1.0 ml 0.1M Tris-HCl, 0.5M NaCl, 10mM glycine, pH 8.0, containing 0.2% bovine serum albumin and 0.1% sodium azide. The characteristics of each lot are tested by electrophoresis and flow cytometry.

储存及稳定性

Store at 2 to 8°C, for up to 6 months. DO NOT FREEZE. Protect from light.

WARNING: The monoclonal reagent solution contains 0.1% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

分析说明

Control
Human peripheral blood lymphocytes, HRIK and Raji cell lines

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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