Merck
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MAB1340

Sigma-Aldrich

Anti-Collagen Type I Antibody, clone C11

ascites fluid, clone C11, Chemicon®

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eCl@ss:
32160702
NACRES:
NA.41

生物来源

mouse

质量水平

抗体形式

ascites fluid

antibody product type

primary antibodies

克隆

C11, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable

同位素/亚型

IgG

NCBI登记号

UniProt登记号

运输

dry ice

target post-translational modification

unmodified

Gene Information

human ... COL1A1(1277)

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此商品
AB745MAB1334AB758
antibody form

ascites fluid

antibody form

purified immunoglobulin (Semi-purified)

antibody form

purified antibody

antibody form

affinity purified immunoglobulin

clone

C11, monoclonal

clone

polyclonal

clone

MMCHABC, monoclonal

clone

polyclonal

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human, bovine

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, immunohistochemistry: suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable, western blot: suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable, radioimmunoassay: suitable

technique(s)

ELISA: suitable, dot blot: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: suitable

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特异性

Recognizes both native and heat denatured human collagen type I. No cross reactivity with albumin fractions of human serum or albumins of other animal species. Less than 5% binding to collagens II and II in ELISA, RIA or immunoblotting.

应用

Detect Collagen Type I using this Anti-Collagen Type I Antibody, clone C11 validated for use in ELISA, IH.
Immunohistochemical staining of 10 μm thick cryostat sections fixed with acetone or ethanol. Staining is similar to that for polyclonal antibodies to interstitial collagen I. Suggested working concentration 10-20 μg/mL. Formaldehyde fixation is not recommended. Does not work with Western blots. Not suitable for use on paraffin embedded sections.

ELISA: Apparent binding constant from titration curves is approximately 0.1nM. Antibody suitable for use in sandwich ELISA for specific determination of collagen I, employed as either a capture antibody or as a second, detecting antibody.

Optimal working dilutions must be determined by the end user.

外形

Immunoglobulin Purified from ascites by ammonium sulfate precipitation and chromatography on DEAE-cellulose. . Lyophilized, reconstitute with 1 mL of distilled water.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

象形图

Exclamation markHealth hazard

警示用语:

Danger

危险分类

Eye Irrit. 2 - Repr. 1B - Skin Irrit. 2

储存分类代码

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 2


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Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence.
Zoumi, A; Yeh, A; Tromberg, BJ
Proceedings of the National Academy of Sciences of the USA null
Patricia Khashayar et al.
3 Biotech, 7(5), 312-312 (2017-09-29)
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Jose M Gonzalez et al.
Scientific reports, 6, 21315-21315 (2016-02-18)
The contractile trabecular meshwork (TM) modulates aqueous humor outflow resistance and intraocular pressure. The primary goal was to visualize and quantify human TM contractile state by analyzing actin polymerization (F-actin) by 2-photon excitation fluorescence imaging (TPEF) in situ. A secondary
Human tendon stem cells better maintain their stemness in hypoxic culture conditions.
Zhang, J; Wang, JH
Testing null
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Ding, Z; Huang, H
BMC Musculoskelet. Disord. null

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