Merck
All Photos(2)

MAB3504-I

Sigma-Aldrich

Anti-Apaf-1 Antibody, clone 13F11

clone 13F11, from rat

Synonym(s):
APAF, APAF-1, APAF1, apoptotic peptidase activating factor 1, apoptotic protease activating factor 1, Apoptotic protease-activating factor 1, CED4, DKFZp781B1145, KIAA0413, Apoptotic protease-activating factor 1
eCl@ss:
32160702
NACRES:
NA.41

生物来源

rat

质量水平

100

抗体形式

purified immunoglobulin

antibody product type

primary antibodies

克隆

13F11, monoclonal

species reactivity

rat, mouse

包装

antibody small pack of 25 μg

technique(s)

western blot: suitable

同位素/亚型

IgG2aκ

NCBI登记号

UniProt登记号

运输

ambient

Gene Information

mouse ... Apaf1(11783)

一般描述

Apoptotic protease-activating factor 1 (UniProt: O88879; also known as Apaf-1) is encoded by the Apaf-1 gene (Gene ID: 11783) in murine species. Apaf-1 contains a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. It is highly expressed in lung and spleen, weakly in brain and kidney and is not detectable in liver. Under basal conditions it is a monomeric protein, but oligomerizes under apoptotic conditions. The monomeric form is auto-inhibited in a closed conformation through a bound ADP at the nucleotide binding site. Under apoptosis signals ADP is exchanged for ATP and its binding to cytochrome c triggers a conformation change where WD repeat region swings out, which allows the NB-ARC domain to rotate and expose the contact areas for oligomerization. Oligomeric form is a heptameric ring that binds to cytochrome c and ATP and is known as the apoptosome. Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 to mature caspase-9, which then activates effector caspases 3, 6, and 7.

特异性

Clone 13F11 specifically detects Apaf-1 in mouse and rat. It targets an epitope within the first 97 amino acids in the N-terminal region.

免疫原

A recombinant fragment corresponding to the first 97 amino acids of mouse Apaf-1.
Epitope: N-terminus

应用

Anti-Apaf-1, clone 13F11, is a rat monoclonal antibody that detects Apaf-1 in mouse and rat. It has been tested for use in Western Blotting.
Research Category
Apoptosis & Cancer
Western Blotting Analysis: 1 ug/mL from a representative lot detected Apaf-1 in mouse bone marrow tissue lysate.

Western Blotting Analysis: A representative lot detected Apaf-1 in Western Blotting applications (Vaughn, A.E., et. al. (2007). Cell Death Differ. 14(5):973-81; Wright, K.M., et. al. (2004). J Cell Biol. 167(2):303-13).

质量

Evaluated by Western Blotting in mouse lymph node tissue lysate.

Western Blotting Analysis: 1 ug/mL of this antibody detected Apaf-1 in mouse lymph node tissue lysate.

目标描述

~141 kDa observed; 141.00 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

联系

Replaces: MAB3504

外形

Protein G purified
Format: Purified
Purified rat monoclonal antibody IgG2a in PBS with 0.02% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(F)

Not applicable

闪点(C)

Not applicable

Certificate of Analysis

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Certificate of Quality

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