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MAB3568

Sigma-Aldrich

Anti-Myosin Antibody, smooth muscle heavy chain, SM1 & SM2, clone ID8

clone ID8 (SMMS-1), Chemicon®, from mouse

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别名:
Anti-CCDC128, Anti-KLRAQ1
eCl@ss:
32160702
NACRES:
NA.41

生物来源

mouse

质量水平

抗体形式

purified immunoglobulin

antibody product type

primary antibodies

克隆

ID8 (SMMS-1), monoclonal

species reactivity

human, bovine, pig, rabbit

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
immunoprecipitation (IP): suitable
western blot: suitable

同位素/亚型

IgG1

NCBI登记号

UniProt登记号

运输

wet ice

target post-translational modification

unmodified

Gene Information

human ... MYH11(4629)

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此商品
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antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

antibody form

purified antibody

clone

ID8 (SMMS-1), monoclonal

clone

N1/5 (SM-M5), monoclonal

clone

MY-32, monoclonal

clone

11C1.3, monoclonal

species reactivity

human, bovine, pig, rabbit

species reactivity

rabbit, bovine, pig, human

species reactivity

rat, chicken, rabbit, mouse, human, bovine, guinea pig, feline

species reactivity

bovine, pig, human

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using porcine tongue, microarray: suitable, western blot: 0.5-1.0 μg/mL using total extract of rabbit skeletal muscle

technique(s)

ELISA: suitable, immunofluorescence: suitable, immunohistochemistry: suitable (paraffin), immunoprecipitation (IP): suitable, western blot: suitable

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应用

This Anti-Myosin Antibody, smooth muscle heavy chain, SM1 & SM2, clone ID8 is validated for use in IP, WB, IC, IH(P) for the detection of Myosin.
Western blot (see application notes below). Suggested blocking buffer is TBS-Tween with 2% BSA. Suggested dilution buffer is TBS-Tween with 0.05% sodium azide. Preferred gel percentage is 5% (see application notes).

Immunohistochemistry on frozen and paraffin embedded tissue sections. Suggested fixation for frozen tissue sections is acetone fix for 6 minutes at room temperature. For formalin fixed paraffin embedded tissue sections: microwave in 0.01M citrate buffer (pH 6.0) for 8-10 minutes (note that all microwaves differ and adjustments may need to be made) follow with enzyme digestion (0.01% pronase for 10 mintues). Suggested blocking agent is fetal bovine serum.

Immunocytochemistry

Immunoprecipitation. Suggested extraction buffer is 20 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Triton X-100, 0.1% SDS, 0.5% deoxycholic acid-NaCl and 0.5 mM PMSF. Final reaction volume is 1 mL and suggested capture agent is agarose conjugated anti-mouse IgG.

Optimal working dilutions must be determined by the end user.

APPLICATION NOTES FOR MAB3568

WESTERN BLOT

To achieve good resolution of myosin heavy chain isoforms with distinct molecular weight (200 - 2004 kDa), the following procedure should be followed: 1). Pyrophoshate extraction buffer for sample preparation (see below); run SDS-PAGE in 5% gel. Important: for better resolution of the MHC bands, use electrophoretic buffer with pH 8.2 (i.e. 0.1 less than standard), and prepare resolving gel (5%) with pH 9.0 (not 8.8 as usual). Also help thorough degasing of the resolving gel mixture (H2O, acrylamide, EDTA, pH 9.0, before (!) adding SDS, TEMED and APS). Run SDS-PAGE longer than after the dye front runs off (use 200 kDa MW markers and let it′s 200 kDa band run at least to the middle of 8X8 gel (using a big size gel (not the mini-gel!) will enhance the quality of MHC band resolution).

Pyrophosphate extraction buffer: (40mM Na4P2O7x10H2O, 1mM MgCl2, 1mM EGTA (add KOH to dissolve EGTA), PMSF, pH 9.5). To extract acto-myosin from tissues/cells, shake minced tissue or cells in cold extraction buffer 1 hr on ice bath (0oC), centrifuge @10,000g for 10 min at +2-8 oC, take supernatant and mix it 1:1 with standard Laemmli sample buffer, boil, run SDS-PAGE in 5% gel (see above).

外形

Format: Purified

分析说明

Control
POSITIVE CONTROL: Smooth muscle (e.g. aterial tunica media). Negative control: any nonmuscle tissue (e.g. arterial tunica adentitial).

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

10 - Combustible liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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