Merck
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MAB5240

Sigma-Aldrich

Anti-Phosphacan Antibody

culture supernatant, Chemicon®

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eCl@ss:
32160702
NACRES:
NA.41

生物来源

mouse

质量水平

抗体形式

culture supernatant

antibody product type

primary antibodies

克隆

monoclonal

species reactivity

chicken

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

同位素/亚型

IgM

UniProt登记号

运输

dry ice

target post-translational modification

unmodified

Gene Information

human ... PTPRZ1(5803)

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此商品
P8874SAB4200690ABN2246
Anti-Phosphacan Antibody culture supernatant, Chemicon®

Sigma-Aldrich

MAB5240

Anti-Phosphacan Antibody

Anti-PTP zeta/Phosphacan from rabbit

Sigma-Aldrich

ABN2246

Anti-PTP zeta/Phosphacan

antibody form

culture supernatant

antibody form

purified immunoglobulin

antibody form

culture supernatant

antibody form

unpurified

clone

monoclonal

clone

122.2, monoclonal

clone

Ber-EP4, monoclonal

clone

polyclonal

species reactivity

chicken

species reactivity

rat

species reactivity

human

species reactivity

rat

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

technique(s)

ELISA: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, western blot: 0.2-0.4 μg/mL using total extract of rat brain

technique(s)

immunofluorescence: 1:500-1:1,000 using human breast adenocarcinoma MCF-7 cell line. , immunohistochemistry: 1:250-1:500 using heat-retrieved formalin-fixed, paraffin-embedded human colon carcinoma sections., immunoprecipitation (IP): suitable

technique(s)

immunohistochemistry: suitable (paraffin)

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一般描述

Phosphacan is a soluble nervous tissue-specific proteoglycan that is thought to regulate axonal outgrowth. It is synthesized by glia and binds to neurons and to the neural cell adhesion molecules tenascin, N-CAM or NG-CAM but not to laminin and fibronectin. Phosphacan acts as a potent inhibitor of cell adhesion and neurite outgrowth.

特异性

Phosphacan (Receptor Tyrosine Phosphatase Beta).

免疫原

Mixture of chicken chondroitin sulfate proteoglycans.

应用

Detect Phosphacan using this Anti-Phosphacan Antibody validated for use in ELISA, WB, IH(P).
Research Category
Neuroscience
Research Sub Category
Growth Cones & Axon Guidance
Western blot: 1:1,000

Samples must be digested with chondroitinase prior to running on SDS gels because undigested phosphacan is too large for most gels. Treatment is at a concentration of chondroitinase of 10U/mL in Tris-HCL pH 8.0. Make tissue or cell extract in 20-50mM Tris pH 7.6-8.0 with 0.15M NaCl in the presence of protease inhibitors. Add 1 microliter of enzyme to 30 microliters of extract and incubate 30 minutes at 37C. Then add SDS sample buffer, heat or boil sample as normal for SDS reducing samples.

Immunohistochemistry: 1:20 Works on cold methanol or paraformaldehyde fixed tissues. Works on paraffin embedded tissues. Chondrotinase treatment of tissue consists of treating mounted tissue section with a solution of PBS plus 0.5U/mL of Chondroitinase ABC for 30 minutes at room temperature prior to blocking and antibody staining.

Immunocytochemistry: not tested

ELISA: Excellent for core and monomer protein.

Optimal working dilutions must be determined by the end user.

外形

Tissue culture supernatant. Liquid. Contains no preservative.

储存及稳定性

Maintain at -20°C in undiluted aliquots up to 6 months. Avoid repeated freeze/thaw cycles.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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