MABC1150

Sigma-Aldrich

Anti-MAGEA3 Antibody, clone 57B

clone 57B, from mouse

别名:
Antigen MZ2-D, Cancer/testis antigen 1.3, CT1.3, MAGE-3 antigen
eCl@ss:
32160702
价格与库存信息目前不能提供

Quality Level

biological source

mouse

antibody product type

primary antibodies

clone

57B, monoclonal

species reactivity

human

application(s)

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

ambient

General description

Melanoma-associated antigen 3 (UniProt: P43357; also known as Antigen MZ2-D, Cancer/testis antigen 1.3, CT1.3, MAGE-3 antigen) is encoded by the MAGEA3 (also known as MAGE3) gene (Gene ID: 4102) in human. MAGE-3 is a member of the melanoma-associated antigen gene family of cancer antigens that are expressed in several neoplastic, but not normal, tissues with the exception of testes. In melanoma cells, it enhances viability and serves as an antigen for cytolytic T lymphocytes. MAGEA3 expression is not reported in kidney tumors, leukemias and lymphomas. MAGEA3 expression is normally limited to early developmental stages. It enhances ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. It is also reported to enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. It is proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex.

Specificity

Clone 57B specifically targets a sequence in the N-terminal region of melanoma associated antigen 3 in human testes.

Immunogen

Epitope: domain of:
His-tagged recombinant human melanoma associated antigen 3 containing the polyhistidine tail.

Application

Anti-MAGEA3 Antibody, clone 57B, Cat. No. MABC1150, detects multiple MAGE-A proteins. Validated for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Research Category
Apoptosis & Cancer
Immunocytochemistry Analysis: A representative lot detected MAGEA3 in Immunocytochemistry applications. (Hofbauer, G.F., et. al. (1997) Am J Pathol. 151(6):1549-53; Landry, C., et. al. (2000). Int J Cancer. 86(6):835-41; Kocher, T., et. al. (1995). Cancer Res. 55(11):2236-9; Juretic, A., et. al. (2003). Lancet Oncol. 4(2):104-9).

Western Blotting Analysis: A representative lot detected MAGEA3 in Western Blot applications. (Kocher, T., et. al. (1995). Cancer Res. 55(11):2236-9; Rimoldi, D., et. al. (2000). Int J Cancer. 86(5):749-51; SHultz-Thater, E., et. al. (2011). Int J Cancer. 129(5):1137-48).

Immunohistochemistry Analysis: A representative lot detected MAGEA3 in Immunohistochemistry applications. (Hofbauer, G.F., et. al. (1997) Am J Pathol. 151(6):1549-53; Aubry, F., et. al. (2001). Cancer. 92(11):2778-85; Cheville, J.C., et. al. (1999). Mod Pathol. 12(10):974-8; Chitale D.A., et. al. (2005). Mod Pathol. 18(1):119-26; Groeper, C., et. al. (2007). Int J Cancer. 120(2):337-43; Juretic, A., et. al. (2003). Lancet Oncol. 4(2):104-9.

Quality

Evaluated by Immunohistochemistry in human testis tissue.

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected MAGEA3 in human testis tissue.

Target description

34.75 kDa calculated.

Physical form

Protein G purified
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Adoptive cancer immunotherapy using DNA-demethylated T helper cells as antigen-presenting cells.
Kirkin AF, Dzhandzhugazyan KN, Guldberg P, Fang JJ, Andersen RS, Dahl C, Mortensen J et al.
Nature Communications, 9(1), 785-785 null

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