MABC1167

Sigma-Aldrich

Anti-P53DINP1 Antibody, clone F8

from rat

别名:
TP53INP1, Tumor protein p53-inducible nuclear protein 1, Stress-induced protein, p53-dependent damage-inducible nuclear protein 1
eCl@ss:
32160702
价格与库存信息目前不能提供

Quality Level

biological source

rat

antibody product type

primary antibodies

clone

monoclonal

species reactivity

mouse, human

packaging

antibody small pack of 25 μL

application(s)

ELISA: suitable
western blot: suitable

isotype

IgG2a

UniProt accession no.

Gene Information

human ... TP53INP1(94241)

General description

Tumor protein p53-inducible nuclear protein 1 (UniProt: Q96A56; also known as Stress-induced protein, p53-dependent damage-inducible nuclear protein 1, p53DINP1) is encoded by the TP53INP1 (also known as P53DINP1, SIP) gene (GeneID: 94241) in human. p53DINP1 is a ubiquitously expressed protein with anti-proliferative and pro-apoptotic properties and is involved in cell stress response. It shuttles between the nucleus and the cytoplasm, depending on cellular stress conditions. It acts as a dual regulator of transcription and autophagy. p53DINP1 acts as a positive regulator of autophagy and in response to cellular stress or upon activation of autophagy, it relocates to autophagosomes where it interacts with autophagosome-associated proteins GABARAP, GABARAPL1/L2, MAP1LC3A/B/C to regulate autophagy. p53DINP1 also acts as an antioxidant and plays a major role in p53/TP53-driven oxidative stress response. It has both a p53/TP53-independent intracellular reactive oxygen species (ROS) regulatory function and a p53/TP53-dependent transcription regulatory function. p53DINP1 is reported to positively regulate p53/TP53 and p73/TP73 and stimulate their capacity to induce apoptosis. In response to double-strand DNA breaks, it promotes p53/TP53 phosphorylation on Ser46 and subsequent apoptosis. It is shown to acts as a tumor suppressor by inducing cell death by an autophagy and caspase-dependent mechanism.

Specificity

Clone F8 detects P53DINP1 (TP53INP1) protein in various human cancer cells.

Immunogen

GST-tagged full length recombinant human Tumor protein p53-inducible nuclear protein 1.

Application

Detect using this rat monoclonal Anti-P53DINP1, clone F8 Antibody, Cat. No. MABC1167-25UL, validated for use in Western Blotting and ELISA.
Western Blotting Analysis: A 1:62.5 dilution from a representative lot detected P53DINP1 in 10 µg of human brain tumor, breast cancer, lymphoma, and pancreatic cancer tissue lysates.
ELISA Analysis: A representative lot detected P53DINP1 in Elisa applications (Saadi, H., et. al. (2013). Results Immunol. 3:51-6).
Western Blotting Analysis: A representative lot detected P53DINP1 in Western Blotting applications (Al Saati, T., et. al. (2013). Am J Pathol. 182(6):1996-2004; Peuget, S., et. al. (2014). Cell Death Differ. 21(7):1107-18; Saadi, H., et. al. (2013). Results Immunol. 3:51-6; Seillier, M., et. al. (2012). Cell Death Differ. 19(9):1525-35).
Research Category
Apoptosis & Cancer

Quality

Evaluated by Western Blotting in human colon tissue lysate.
Western Blotting Analysis: A 1:62.5 dilution of this antibody detected P53DINP1 in 10 µg of human colon tissue lysate.

Physical form

Protein G purified
Format: Purified
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

分析证书
原产地证书 (CofO)

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