Tumor protein p53-inducible nuclear protein 1 (UniProt: Q96A56; also known as Stress-induced protein, p53-dependent damage-inducible nuclear protein 1, p53DINP1) is encoded by the TP53INP1 (also known as P53DINP1, SIP) gene (GeneID: 94241) in human. p53DINP1 is a ubiquitously expressed protein with anti-proliferative and pro-apoptotic properties and is involved in cell stress response. It shuttles between the nucleus and the cytoplasm, depending on cellular stress conditions. It acts as a dual regulator of transcription and autophagy. p53DINP1 acts as a positive regulator of autophagy and in response to cellular stress or upon activation of autophagy, it relocates to autophagosomes where it interacts with autophagosome-associated proteins GABARAP, GABARAPL1/L2, MAP1LC3A/B/C to regulate autophagy. p53DINP1 also acts as an antioxidant and plays a major role in p53/TP53-driven oxidative stress response. It has both a p53/TP53-independent intracellular reactive oxygen species (ROS) regulatory function and a p53/TP53-dependent transcription regulatory function. p53DINP1 is reported to positively regulate p53/TP53 and p73/TP73 and stimulate their capacity to induce apoptosis. In response to double-strand DNA breaks, it promotes p53/TP53 phosphorylation on Ser46 and subsequent apoptosis. It is shown to acts as a tumor suppressor by inducing cell death by an autophagy and caspase-dependent mechanism.
Clone F8 detects P53DINP1 (TP53INP1) protein in various human cancer cells.
GST-tagged full length recombinant human Tumor protein p53-inducible nuclear protein 1.
Detect using this rat monoclonal Anti-P53DINP1, clone F8 Antibody, Cat. No. MABC1167-25UL, validated for use in Western Blotting and ELISA.
Western Blotting Analysis: A 1:62.5 dilution from a representative lot detected P53DINP1 in 10 µg of human brain tumor, breast cancer, lymphoma, and pancreatic cancer tissue lysates.
ELISA Analysis: A representative lot detected P53DINP1 in Elisa applications (Saadi, H., et. al. (2013). Results Immunol. 3:51-6).
Western Blotting Analysis: A representative lot detected P53DINP1 in Western Blotting applications (Al Saati, T., et. al. (2013). Am J Pathol. 182(6):1996-2004; Peuget, S., et. al. (2014). Cell Death Differ. 21(7):1107-18; Saadi, H., et. al. (2013). Results Immunol. 3:51-6; Seillier, M., et. al. (2012). Cell Death Differ. 19(9):1525-35).
Apoptosis & Cancer
Evaluated by Western Blotting in human colon tissue lysate.
Western Blotting Analysis: A 1:62.5 dilution of this antibody detected P53DINP1 in 10 µg of human colon tissue lysate.
Protein G purified
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Concentration: Please refer to lot specific datasheet.
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