MABC1560

Sigma-Aldrich

Anti-REST Antibody, clone 2D9.1

clone 2D9.1, from mouse

别名:
RE1-silencing transcription factor, Neural-restrictive silencer factor, X2 box repressor
eCl@ss:
32160702
价格与库存信息目前不能提供

Quality Level

biological source

mouse

antibody product type

primary antibodies

clone

2D9.1, monoclonal

species reactivity

human

packaging

antibody small pack of 25 μg

application(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

isotype

IgG1κ

NCBI accession no.

UniProt accession no.

shipped in

ambient

General description

RE1-silencing transcription factor (UniProt: Q13127; also known as REST, Neural-restrictive silencer factor, X2 box repressor) is encoded by the REST (also known as NRSF, XBR) gene (Gene ID: 5978) in human. REST is a transcriptional repressor that acts by binding a DNA sequence element called the neuron-restrictive silencer element (NRSE). It is ubiquitously expressed and higher expression is found in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary. The protein is also found in undifferentiated neuronal progenitor cells and it is believed that it acts as a master negative regular of neurogenesis. REST restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. REST also mediates repression by recruiting the BHC complex at RE1/NRSE sites, which acts by deacetylating and demethylating specific sites on histones and acting as a chromatin modifier. REST is shown to contain nine zinc finger domains, but a C-terminally truncated form is also produced by alternative splicing. This variant, REST4, contains five of the zinc-finger domains and has weaker DNA binding capacity. Mutations in REST gene can lead to pediatric malignancy of the kidney which occurs due to uncontrolled multiplication of renal stem, stromal, and epithelial cells.

Specificity

Clone 2D9.1 detects RE1-silencing transcription factor in human cells. It targets an epitope within 297 amino acids from the C-terminal region.

Immunogen

GST/His-Tagged recombinant fragment corresponding to 297 amino acids from the C-terminal region of human RE1-silencing transcription factor (REST).

Application

Anti-REST, clone 2D9.1, Cat. No. MABC1560, s a mouse monoclonal antibody that detects REST and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.
Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected REST in human tonsil and human small intestine tissues.

Quality

Evaluated by Western Blotting in HeLa cell nuclear extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected REST in 10 µg of HeLa cell nuclear extract.

Target description

~180 kda observed; 121.87 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Protein G purified
Format: Purified

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门

社交媒体

LinkedIn icon
Twitter icon
Facebook Icon
Instagram Icon

Merck

科研、开发、生产。

作为生命科学行业的全球领先供应商,我们致力于为科研、生物技术开发和生产,以及制药药物疗法开发和生产提供各类解决方案和服务。

© 2021年版权归德国达姆施塔特默克集团(Merck KGaA)及/或其附属公司所有。版权所有。

未经许可,严禁复制本网站上的任何资料。